AdipoGen Life Sciences

anti-APRIL (human), mAb (blocking) (Mahya-1) (preservative free)

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Product Details
Synonyms A Proliferation Inducing Ligand; TNFSF13; CD256; TALL-2
Product Type Monoclonal Antibody
Properties
Clone Mahya-1
Isotype Mouse IgG1κ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant human APRIL (aa 98-233).
Label/Conjugates Preservative Free
Application

ELISA
Immunoprecipitation: (1:200)
Functional Application: Inhibits the binding of human APRIL to BCMA and TACI.

Crossreactivity Human
Specificity

Recognizes human APRIL. Does not recognize mouse APRIL or mouse and human BAFF.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL-test; Lonza)
Concentration 1mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Mouse IgG1 Isotype Control (preservative free)

Other Product Data

Uniprot Link O75888: April (human)

Accession Number O75888
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

APRIL is a secreted cytokine, belonging to the TNF superfamily that binds to transmembrane activator and CAML interactor (TACI), B cell maturation antigen (BCMA) and can interact with carbohydrate side chains of heparan sulfate proteoglycans (HSPG) that may trigger cross-linking. APRIL maintains B cell homeostasis by acting at a later stage, modulating the function and survival of antigen-experienced B cells. APRIL (as well as BAFF) stimulates class-switch recombination (CSR), hence contributes to shaping humoral effector mechanisms. With regards to humoral memory, APRIL is involved in the establishment and survival of the long-lived plasma cell (LLPC) pool in the bone marrow (BM). Recently, APRIL has been shown to confer atheroprotection via binding to heparan sulfate (HS) chains of heparan-sulfate proteoglycan 2 (HSPG2), which limits LDL retention, macrophage accumulation and necrotic core formation. BAFF and APRIL are implicated in several human autoimmune diseases with autoreactive B cell involvement, including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SS), IgA nephropathy (IgAN), and rheumatoid arthritis (RA). APRIL might also function in enhancing the proliferation of some tumor cells, especially B cell malignancies. BAFF and APRIL contribute to maintaining IgG, IgA, and IgM plasma cells in the bone marrow (BM), spleen and intestine and rely mainly on three ligand/receptor pairs: APRIL-BCMA, BAFF-TACI, and APRIL-TACI. APRIL plays an important role in B cell survival and proliferation, so blocking it can modulate immune responses. APRIL blocking antibodies can be useful for vaccine research, particularly to assess how APRIL inhibition affects normal immune responses to vaccination.

Product References
  1. APRIL limits atherosclerosis by binding to heparan sulfate proteoglycans: D. Tsiantoulas, et al.; Nature 597, 92 (2021)
  2. Polarized Secretion of APRIL by the Tonsil Epithelium Upon Toll-Like Receptor Stimulation: N. Sturm, et.al.; Front. Immunol. 12, 715724 (2021)
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