anti-APRIL (mouse), mAb (rec.) (blocking) (Apry-1-1)

CHF 480.00
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AG-27B-0001-C100100 µgCHF 480.00
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Product Details
Synonyms A Proliferation Inducing Ligand; TNFSF13; CD256; TALL-2
Product Type Recombinant Antibody
Properties
Clone Apry-1-1
Isotype Mouse IgG2bλ
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Mouse APRIL (aa 98-232).
Application

ELISA
Immunoprecipitation: (1:200)
Functional: Inhibits the binding of mouse APRIL to BCMA and TACI. Works in vitro and in vivo. For additional information see References 1 & 5.

Crossreactivity Mouse
Specificity

Recognizes mouse APRIL. Does not recognize mouse BAFF.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL-test; Lonza)
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Other Product Data

APRIL (mouse) monoclonal antibody (recombinant) (blocking) (APRY-1-1) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes.

Anti-APRIL (mouse) Monoclonal Antibody (recombinant) (Blocking) (APRY-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Product References
  1. Production of the plasma-cell survival factor APRIL peaks in myeloid precursor cells from human bone marrow: T. Matthes, et al.; Blood 118, 1838 (2011)
  2. Pathogenic role of a proliferation-inducing ligand (APRIL) in murine IgA nephropathy: Y.G. Kim, et al.; PLoS One 10, e0137044 (2015)
  3. The B cell-stimulatory cytokines BLyS and APRIL are elevated in human periodontitis and are required for B cell-dependent bone loss in experimental murine periodontitis: T. Abe, et al.; J. Immunol. 195, 1427 (2015)
  4. Splenic marginal zone granulocytes acquire an accentuated neutrophil B-cell helper phenotype in chronic lymphocytic leukemia: M. Gaetjen, et al.; Cancer Res. 76, 5253 (2016)
  5. A mouse model of systemic lupus erythematosus responds better to soluble TACI than to soluble BAFFR, correlating with depletion of plasma cells: P. Haselmayer, et al.; Eur. J. Immunol. 47, 1075 (2017) (+Supplement)
  6. Plasma cell output from germinal centers is regulated by signals from Tfh and stromal cells: Y. Zhang, et al.; J. Exp. Med. 215, (2018)
  7. Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis: H.-Y. Huang, et al.; PNAS 115, E6826 (2018)
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