AdipoGen Life Sciences

anti-CD3 (human), mAb (TR66) (preservative free)

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AG-20B-0087PF-C100100 µgCHF 190.00

AG-20B-0087PF-C500500 µgCHF 760.00

Figure 1: anti-CD3 (human), mAb (TR66) (preservative-free) (Prod. No. AG-20B-0087PF) activates T cells. Methods: T cells (10E4 cells) are incubated with PBS (Cells) with an anti-CD3 from a Competitor at 30 ng/ml or with different concentrations of TR66 (#AG-20B-0087PF) as indicated in the figure. As a readout of T cell activation, IFN-γ is measured using an ELISA, and the concentration of IFN-γ per 10E4 T cells is shown. Courtesy of Markus Maeurer, Immunotherapy Cell Center Champalimaud Foundation, Portugal.

Specifications / Handling

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Product Details
Synonyms	CD3ε; CD3e; T Cell Surface Glycoprotein CD3 epsilon Chain; T Cell Surface Antigen T3/Leu-4 epsilon Chain; T3E
Product Type	Monoclonal Antibody
Properties
Clone	TR66
Isotype	Mouse IgG1
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Cloned human helper T cell line.
Application	Flow Cytometry Immunocytochemistry Immunoprecipitation Western Blot Functional Application: Activates T cells in vivo and in vitro, together with a costimulatory signal and cytokine support.
Crossreactivity	Human
Specificity	Recognizes the ε-chain of CD3.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS.
Isotype Negative Control	Mouse IgG1 Isotype Control (preservative free)
Other Product Data	Uniprot: CD3 (human)
Accession Number	P07766
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

CD3 is a multi-subunit protein complex expressed on the surface of T lymphocytes as part of the T cell receptor (TCR) complex. It plays a central role in T cell activation by transmitting signals from the TCR upon antigen recognition. Because of its consistent expression across all mature T cells, CD3 serves as a key pan-T cell marker in immunology research. Anti-CD3 antibodies are widely used to study T cell biology and immune signaling. They are used to identify and characterize T cells or activate T cells in vitro by crosslinking the TCR/CD3 complex, enabling functional assays of proliferation and cytokine production. The versatile applications make anti-CD3 antibodies essential tools in immunology, cancer immunotherapy research and studies of autoimmune diseases.

Product-specific References

The use of hybrid hybridomas to target human cytotoxic T lymphocytes: A. Lanzavecchia & D. Scheidegger; Eur. J. Immunol. 17, 105 (1987) [PMID: 3102250]
Efficiency of T cell triggering by anti-CD3 monoclonal antibodies (mAb) with potential usefulness in bispecific mAb generation: N. Jacobs, et al.; Cancer Immunol. Immunother. 44, 257 (1997) [PMC11037629]
FLIP prevents apoptosis induced by death receptors but not by perforin/granzyme B, chemotherapeutic drugs, and gamma irradiation: T. Kataoka, et al.; J. Immunol. 161, 3936 (1998) [PMID: 9780161]
T lymphocyte costimulation mediated by reorganization of membrane microdomains: A. Viola, et al.; Science 283, 680 (1999) [PMID: 9924026]
Optimizing anti-CD3 affinity for effective T cell targeting against tumor cells: N. Bortoletto, et al; Eur. J. Immunol. 32, 3102 (2002) [PMID: 12385030]
T cell fitness determined by signal strength: A.V. Gett, et al.; Nat. Immunol. 4, 355 (2003) [PMID: 12640450]
Chemokine receptor expression identifies Pre-T helper (Th)1, Pre-Th2, and nonpolarized cells among human CD4+ central memory T cells: L. Rivino, et al.; J. Exp. Med. 200, 725 (2004) [PMC2211963]
Coligation of the Hepatitis C Virus Receptor CD81 with CD28 Primes Naive T Lymphocytes to Acquire Type 2 Effector Function: A. Serra, et al.; J. Immunol. 181, 174 (2008) [PMID: 18566382]
CD45RC isoform expression identifies functionally distinct T cell subsets differentially distributed between healthy individuals and AAV patients: L. Ordonez, et al.; PLoS One 4, e5287 (2009) [PMC2668071]
Design, selection and optimization of an anti-TRAIL-R2/anti-CD3 bispecific antibody able to educate T cells to recognize and destroy cancer cells: A. Satta, et al.; MAbs 10, 1084 (2018) [PMC6204841]
Tumor-induced senescent T cells promote the secretion of pro-inflammatory cytokines and angiogenic factors by human monocytes/macrophages through a mechanism that involves Tim-3 and CD40L: M.C. Ramello, et al.; Cell Death Dis. 5, e1507 (2014) [PMC4260722]
ERK phosphorylation and miR-181a expression modulate activation of human memory TH17 cells: F. Mele, et al.; Nat. Commun. 6, 6431 (2015) [PMID: 25775432]
The Interplay Between Neutrophils and CD8+ T Cells Improves Survival in Human Colorectal Cancer: V. Governa, et al.; Clin. Cancer Res. 23, 3847 (2017) [PMID: 28108544]
An immunoregulatory and tissue-residency program modulated by c-MAF in human TH17 cells: D. Aschenbrenner, et al.; Nat. Immunol. 19, 1126 (2018) [PMC6402560]
An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes: C. Leoni, et al.; PLoS One 16, e0247232 (2021) [PMC7891767]
RFX transcription factors control a miR-150/PDAP1 axis that restrains the proliferation of human T cells: M. Chirichella, et al.; PLoS Biol. 20, e3001538 (2022) [PMC8865640]
Proteomics of immune cells from liver tumors reveals immunotherapy targets: F.P. Canale, et al.; Cell Genom. 3, 100331 (2023) [PMC10300607]
Activation of endogenous retroviruses and induction of viral mimicry by MEK1/2 inhibition in pancreatic cancer: A. Cortesi, et al.; Sci. Adv. 10, eadk5386 (2024) [PMC10971493]
FCRL3 is an immunoregulatory receptor that restrains the activation of human memory T lymphocytes: N. Bianchi, et al.; J. Exp. Med. 223, e20242474 (2026) [PMC12524113]

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