AdipoGen Life Sciences

anti-PLXDC2, mAb (Bonny-2)

As low as CHF 170.00

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AG-20B-0089-TRIAL25 µgCHF 170.00

AG-20B-0089-C100100 µgCHF 410.00

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Figure 1: Detection of mouse PLXDC2 with anti-PLXDC2, mAb (Bonny-2) (Prod. No. AG-20B-0089).
Method: 3x10^6 C1498 lymphoblasts (A) or transduced with mouse PLXDC2 (B) are blocked with FcR Mouse Blocker for 10 min at RT, then stained with anti-PLXDC2, mAb (Bonny-2) (diluted at 1/100) in 100µl of FACS buffer (PBS, 2% FCS, P/S, 1mM EDTA) for 20 min at 4°C. The cells are incubated for 15 min at 4°C with a secondary antibody anti-mouse IgG-APC diluted at 1/100 in 50µl of FACS buffer. The cells are then stained for surface markers following a standard protocol. (Picture courtesy of Laurie Bonneaux, Prof. Werner Held lab, University of Lausanne)

Specifications / Handling

More Information
Product Details
Synonyms	Plexin Domain-containing Protein 2; Tumor Endothelial Marker 7-related Protein
Product Type	Monoclonal Antibody
Properties
Clone	Bonny-2
Isotype	Mouse IgG1κ
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Recombinant mouse PLXDC2.
Application	Flow Cytometry: (1:200) ELISA: (1:2000) Using PLXDC2 and CD150 antibodies together in flow cytometry allows easy hematopoietic stem cell (HSC) isolation.
Crossreactivity	Human Mouse
Specificity	Recognizes endogenous human and mouse PLXDC2 with the same activity.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control	Mouse IgG2a Isotype Control
Accession Number	Q6UX71 (human) and Q9DC11 (mouse)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Plexin domain containing 2 (PLXDC2) is a nidogen/plexin homology transmembrane protein encoded by PLXDC2. PLXDC2 is specifically expressed on the surface of macrophages but not neutrophils, T cells or NK cells. PLXDC2 has several functions: i) in the brain, it is involved in the proliferation and differentiation of neural progenitors during the development of the nervous system; ii) in the immune system, PLXDC2 on macrophages binds to secreted PTEN (phosphatase and tensin homolog) to sequentially activate JAK2-STAT1 signaling, reprogram macrophages and drive antitumor immunity. Aberrant expression of PLXDC2 in different cancers has been reported. PLXDC2, together with its homolog PLXDC1, also seems to be the receptor for pigment epithelium-derived factor (PEDF) in endothelial cells. The membrane protein PLXDC2 is highly expressed on human and mouse HSCs. Using this new marker together with CD150 in flow cytometry allows easy hematopoietic stem cell (HSC) isolation. A new protocol has been described, which is much more efficient compared to the conventional positive and negative markers protocols (CD34+CD38-CD90+CD45RA-CD49f+) that are used at the moment.

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