anti-PLXDC2, mAb (Bonny-2)

Plexin domain containing 2 (PLXDC2) is a nidogen/plexin homology transmembrane protein encoded by PLXDC2. PLXDC2 is specifically expressed on the surface of macrophages but not neutrophils, T cells or NK cells. PLXDC2 has several functions: i) in the brain, it is involved in the proliferation and differentiation of neural progenitors during the development of the nervous system; ii) in the immune system, PLXDC2 on macrophages binds to secreted PTEN (phosphatase and tensin homolog) to sequentially activate JAK2-STAT1 signaling, reprogram macrophages and drive antitumor immunity. Aberrant expression of PLXDC2 in different cancers has been reported. PLXDC2, together with its homolog PLXDC1, also seems to be the receptor for pigment epithelium-derived factor (PEDF) in endothelial cells. The membrane protein PLXDC2 is highly expressed on human and mouse HSCs. Using this new marker together with CD150 in flow cytometry allows easy hematopoietic stem cell (HSC) isolation. A new protocol has been described, which is much more efficient compared to the conventional positive and negative markers protocols (CD34+CD38-CD90+CD45RA-CD49f+) that are used at the moment.