Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
anti-Bmf, mAb (9G10)
430
CHF
CHF 430.00
In stock
AG-20T-0130-C100100 µgCHF 430.00
| Product Details | |
|---|---|
| Synonyms | Bcl-2-modifying Factor |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 9G10 |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Recombinant mouse Bmf. |
| Application |
Flow Cytometry: (overexpressed in cell lines) |
| Crossreactivity |
Human Mouse |
| Specificity |
Recognizes human and mouse Bmf. Detects several cross-reactivity bands of unknown identity in mice by western blot. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Bmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3), and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2), which may be important for sensing intracellular damage and triggering apoptosis.
Product References
- Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science 293, 1829 (2001)
- Chemokine receptors CXCR4 and CCR7 promote metastasis by preventing anoikis in cancer cells: M. Kochetkova, et al.; Cell Death Differ. 16, 664 (2009)
- Suppression of B cell lymphomagenesis by the BH3-only proteins Bmf and Bad: A. Frenzel, et al.; Blood 115, 995 (2010)
- BH3-only protein Bmf mediates apoptosis upon inhibition of CAP-dependent protein synthesis: F. Grespi, et al.; Cell Death Diff. 17, 1672 (2010)
- Vorinostat-Induced Apoptosis in Mantle Cell Lymphoma Is Mediated by Acetylation of Proapoptotic BH3-Only Gene Promoters: S. Xargay-Torrent, et al.; Clin. Cancer Res. 17, 3956 (2011)
- Haematopoietic stem cell survival and transplantation efficacy is limited by the BH3-only proteins Bim and Bmf: V. Labi, et al.; EMBO Mol. Med. 5, 122 (2013)
Related Products
