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AdipoGen Life Sciences
anti-Bmf (mouse/rat), mAb (17A9)
430
CHF
CHF 430.00
In stock
AG-20T-0131-C100100 µgCHF 430.00
Figure 1: Western blot using anti-Bmf (mouse/rat), mAb (17A9) (Prod. No. AG-20T-0131) detecting endogenous Bmf in mouse spleen, lymph node and thymus as two bands of ~25 and ~30 kDa, but not in tissues from two knock-out Bmf strains del 372 and del 376.
Figure 2: Immunoprecipitation of endogenous Bmf from mouse spleen cells using MAb to Bmf (17A9) (Prod. No. AG-20T-0131).
Figure 3: Flow cytometry data of overexpressed EE-tagged Bmf in 293T cells, fixed with 1% PFA, permeabilized with saponin and stained intracellularly with anti-Bmf (mouse/rat), mAb (17A9) (Prod. No. AG-20T-0131), control or positive control anti-EE.
| Product Details | |
|---|---|
| Synonyms | Bcl-2-modifying Factor |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 17A9 |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 and aa 112-126 of mouse Bmf. |
| Application |
ELISA |
| Crossreactivity |
Mouse Rat |
| Specificity |
Recognizes an epitope within the peptide sequence aa 13-27 of mouse and rat Bmf. Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL. Does not cross-react with human Bmf. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Bmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3), and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2), which may be important for sensing intracellular damage and triggering apoptosis.
Product References
- Apoptosis-based dual molecular targeting by INNO-406, a second-generation Bcr-Abl inhibitor, and ABT-737, an inhibitor of antiapoptotic Bcl-2 proteins, against Bcr-Abl-positive leukemia: J. Kuroda, et al.; Cell Death Differ. 14, 1667 (2007)
- Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008)
- Suppression of B cell lymphomagenesis by the BH3-only proteins Bmf and Bad: A. Frenzel, et al.; Blood 115, 995 (2010)
- BH3-only protein Bmf mediates apoptosis upon inhibition of CAP-dependent protein synthesis: F. Grespi, et al.; Cell Death Diff. 17, 1672 (2010)
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