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AdipoGen Life Sciences
anti-Apaf-1 (mouse/rat), mAb (13F11)
390
CHF
CHF 390.00
In stock
AG-20T-0133-C100100 µgCHF 390.00
Figure 1: Western blot analysis using anti-Apaf-1 (mouse/rat), MAb (13F11) (AG-20T-0133) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.
Figure 2: Immunoprecipitation on mouse thymocytes using anti-Apaf-1 (mouse/rat), MAb (13F11) (AG-20T-0133).
| Product Details | |
|---|---|
| Synonyms | Apoptosis Protease-activating Factor 1 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 13F11 |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain. |
| Application |
ELISA |
| Crossreactivity |
Mouse Rat |
| Specificity |
Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
Product References
- Histone deacetylase activity regulates apaf-1 and caspase 3 expression in the developing mouse retina: D.M. Wallace, et al.; Invest. Ophthalmol. Vis. Sci. 47, 2765 (2006)
- Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues: C.E. Johnson, et al.; PNAS 104, 20820 (2007)
- Chromatin modification of Apaf-1 restricts the apoptotic pathway in mature neurons: K.M. Wright, et al.; J. Cell Biol. 179, 825 (2007)
- Inhibition of apoptosome formation by suppression of Hsp90beta phosphorylation in tyrosine kinase-induced leukemias: M. Kurokawa, et al.; Mol. Cell Biol. 28, 5494 (2008)
- Suppression of the intrinsic apoptosis pathway by synaptic activity: F. Leveille, et al.; J. Neurosci. 30, 2623 (2010)
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