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AdipoGen Life Sciences
anti-Apaf-1 (human), mAb (2E12)
390
CHF
CHF 390.00
In stock
AG-20T-0132-C100100 µgCHF 390.00
Figure 1: Western blot analysis using anti-Apaf-1 (human), MAb (2E12) (AG-20T-0132) on several human cell lines and one monkey cell line (COS).
Figure 2: Immunohistochemical staining of Apaf-1 using anti-Apaf-1 (human), mAb (2E12) (Prod. No. AG-20T-0132) in formalin-fixed and paraffin-embedded (FFPE) human kidney tissue (10µg/ml).
| Product Details | |
|---|---|
| Synonyms | Apoptosis Protease-activating Factor 1 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 2E12 |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains. |
| Application |
ELISA |
| Crossreactivity | Human |
| Specificity |
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
Product References
- Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1: K. Moriishi, et al.; PNAS 96, 9683 (1999)
- Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 49, 623 (2000)
- Apaf-1 is a transcriptional target for E2F and p53: M.C. Moroni, et al.; Nat. Cell Biol. 3, 552 (2001)
- Is Apaf-1 expression frequently abrogated in melanoma?: J.D. Allen, et al.; Cell Death Differ. 12, 680 (2005)
- Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues: C.E. Johnson, et al.; PNAS 104, 20820 (2007)
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