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AdipoGen Life Sciences
anti-Apaf-1, mAb (18H2)
390
CHF
CHF 390.00
In stock
AG-20T-0134-C100100 µgCHF 390.00
Figure 1: Western blot analysis using anti-Apaf-1, mAb (18H2) (AG-20T-0134) on lysates from mouse cell lines.
Figure 2: Immunoprecipitation on mouse thymocytes using anti-Apaf-1, mAb (18H2) (AG-20T-0134).
| Product Details | |
|---|---|
| Synonyms | Apoptosis Protease-activating Factor 1 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 18H2 |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain. |
| Application |
ELISA |
| Crossreactivity |
Human Mouse |
| Specificity |
Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
Product References
- Apaf-1 is a transcriptional target for E2F and p53: M.C. Moroni, et al.; Nat. Cell Biol. 3, 552 (2001)
- Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis: A.G. Martin & H.O. Fearnhead; J. Biol. Chem. 277, 50834 (2002)
- Apaf-1 and caspase-9 accelerate apoptosis, but do not determine whether factor-deprived or drug-treated cells die: P.G. Ekert, et al.; J. Cell. Biol. 165, 835 (2004)
- Is Apaf-1 expression frequently abrogated in melanoma?: J.D. Allen, et al.; Cell Death Differ. 12, 680 (2005)
- The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage: D. De Zio, et al.; Cell Death Differ. 18, 516 (2011)
- Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell free and cell-based systems: A.R. Noori, et al.; Arch. Biochem. Biophys. 642, 46 (2018)
- The Lumiptosome, an engineered luminescent form of the apoptosome can report cell death by using the same Apaf-1 dependent pathway: E.S. Hosseini, et al.; J. Cell Sci. 133, 242636 (2020)
- Loss of WD2 subdomain of Apaf-1 forms an apoptosome structure which blocks activation of caspase-3 and caspase-9: A.R. Noori, et al.; Biochim. 180, 23 (2021)
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