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AdipoGen Life Sciences
anti-Caspase-8 (mouse), mAb (3B10)
350
CHF
CHF 350.00
In stock
AG-20T-0138-C100100 µgCHF 350.00
Figure 1: Flow cytometry data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using anti-caspase-8 mAbs (3B10) (Prod. No. AG-20T-0138), anti-FLAG or control. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
Figure 2: Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.
Figure 3: Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.
Figure 4: Western blot using anti-Caspase-8 (mouse), mAb (3B10) (Prod. No. AG-20T-0138) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p20 subunit. Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue.
| Product Details | |
|---|---|
| Synonyms | Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 3B10 |
| Isotype | Rat IgG1κ |
| Immunogen/Antigen | Recombinant mouse caspase-8 (p20 subunit). |
| Application |
ELISA: (recombinant protein) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
Product References
- Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly, et al.; Cell Death Differ. 11, 724 (2004)
- Stimulation of Toll-like receptor 3 and 4 induces interleukin-1 maturation by caspase-8: J. Maelfait, et al.; J. Exp. Med. 205, 1967 (2008)
- Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium: M. Shackleton, et al.; Dev. Dyn. 238, 1010 (2009)
- Caspase-8 mediates mitochondrial release of pro-apoptotic proteins in a manner independent of its proteolytic activity in apoptosis induced by the protein synthesis inhibitor acetoxycycloheximide in human leukemia Jurkat cells: K. Kadohara, et al.; J. Biol. Chem. 284, 5478 (2009)
- Cytotoxic T lymphocyte perforin and Fas ligand working in concert even when Fas ligand lytic action is still not detectable: D. Hassin, et al.; Immunology 133, 190 (2011)
- Necroptotic signaling is primed in Mycobacterium tuberculosisinfected macrophages, but its pathophysiological consequence in disease is restricted: M.S. Stutz, et al.; Cell Death Diff. 25, 951 (2018)
- IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death: R. Karki, et al.; J. Immunol. 204, 2514 (2020)
- COVID-19 cytokines and the hyperactive immune response: Synergism of TNF-α and IFN-γ in triggering inflammation, tissue damage, and death: R. Karki, et al.; bioRxiv (Preprint) (2020)
- The nonreceptor tyrosine kinase SYK drives caspase-8/NLRP3 inflammasome-mediated autoinflammatory osteomyelitis: T.K. Dasari, et al.; 295, 3394 (2020)
- RIPK1 Distinctly Regulates Yersinia-Induced Inflammatory Cell Death, PANoptosis: E.K. Subbarao Malireddi, et al.; ImmunoHorizons 4, 789 (2020)
- Synergism of TNF-a and IFN-g Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARSCoV-2 Infection and Cytokine Shock Syndromes: R. Karki, et al.; Cell 184, 149 (2021)
- Hierarchical Cell Death Program Disrupts the Intracellular Niche Required for Burkholderia thailandensis Pathogenesis: D.E. Place, et al.; . mBio 12, e01059 (2021)
- DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon response: S. Kesavardhana, et al.; J. Biol. Chem. 296, 100579 (2021)
- NLRC4 Deficiency Leads to Enhanced Phosphorylation of MLKL and Necroptosis: B. Sundaram, et al.; Immunohorizons 6, 243 (2022)
- Single cell analysis of PANoptosome cell death complexes through an expansion microscopy method: Y. Wang, et al.; Cell Mol. Life Sci. 79, 531 (2022)
- Molecular mechanism of RIPK1 and caspase-8 in homeostatic type I interferon production and regulation: Y. Wang, et al.; Cell Rep. 41, 111434 (2022)
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