AdipoGen Life Sciences

anti-Caspase-8 (mouse), mAb (3B10)

CHF 350.00
In stock
AG-20T-0138-C100100 µgCHF 350.00
More Information
Product Details
Synonyms Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease
Product Type Monoclonal Antibody
Properties
Clone 3B10
Isotype Rat IgG1κ
Immunogen/Antigen Recombinant mouse caspase-8 (p20 subunit).
Application

ELISA: (recombinant protein)
Flow Cytometry: (overexpressed in cell lines)
Immunocytochemistry: (excellent, see literature reference 2)
Immunohistochemistry
Western Blot: (1-2μg/ml)
Optimal conditions must be determined individually for each application.

Crossreactivity Mouse
Specificity

Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified (sepharose column).
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rat IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.

Product References
  1. Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly, et al.; Cell Death Differ. 11, 724 (2004)
  2. Stimulation of Toll-like receptor 3 and 4 induces interleukin-1 maturation by caspase-8: J. Maelfait, et al.; J. Exp. Med. 205, 1967 (2008)
  3. Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium: M. Shackleton, et al.; Dev. Dyn. 238, 1010 (2009)
  4. Caspase-8 mediates mitochondrial release of pro-apoptotic proteins in a manner independent of its proteolytic activity in apoptosis induced by the protein synthesis inhibitor acetoxycycloheximide in human leukemia Jurkat cells: K. Kadohara, et al.; J. Biol. Chem. 284, 5478 (2009)
  5. Cytotoxic T lymphocyte perforin and Fas ligand working in concert even when Fas ligand lytic action is still not detectable: D. Hassin, et al.; Immunology 133, 190 (2011)
  6. Necroptotic signaling is primed in Mycobacterium tuberculosisinfected macrophages, but its pathophysiological consequence in disease is restricted: M.S. Stutz, et al.; Cell Death Diff. 25, 951 (2018)
  7. IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death: R. Karki, et al.; J. Immunol. 204, 2514 (2020)
  8. COVID-19 cytokines and the hyperactive immune response: Synergism of TNF-α and IFN-γ in triggering inflammation, tissue damage, and death: R. Karki, et al.; bioRxiv (Preprint) (2020)
  9. The nonreceptor tyrosine kinase SYK drives caspase-8/NLRP3 inflammasome-mediated autoinflammatory osteomyelitis: T.K. Dasari, et al.; 295, 3394 (2020)
  10. RIPK1 Distinctly Regulates Yersinia-Induced Inflammatory Cell Death, PANoptosis: E.K. Subbarao Malireddi, et al.; ImmunoHorizons 4, 789 (2020)
  11. Synergism of TNF-a and IFN-g Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARSCoV-2 Infection and Cytokine Shock Syndromes: R. Karki, et al.; Cell 184, 149 (2021)
  12. Hierarchical Cell Death Program Disrupts the Intracellular Niche Required for Burkholderia thailandensis Pathogenesis: D.E. Place, et al.; . mBio 12, e01059 (2021)
  13. DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon response: S. Kesavardhana, et al.; J. Biol. Chem. 296, 100579 (2021)
  14. NLRC4 Deficiency Leads to Enhanced Phosphorylation of MLKL and Necroptosis: B. Sundaram, et al.; Immunohorizons 6, 243 (2022)
  15. Single cell analysis of PANoptosome cell death complexes through an expansion microscopy method: Y. Wang, et al.; Cell Mol. Life Sci. 79, 531 (2022)
  16. Molecular mechanism of RIPK1 and caspase-8 in homeostatic type I interferon production and regulation: Y. Wang, et al.; Cell Rep. 41, 111434 (2022)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.