AdipoGen Life Sciences

anti-Adiponectin (rat), pAb

CHF 390.00

In stock

AG-25A-0005-C100100 µgCHF 390.00

Figure 1: Immunohistochemical staining of rat hypothalamus using anti-adiponectin (rat), pAb (Prod. No. AG-25A-0005) at 1:1'000 dilution.

Figure 2: Western blot analysis using anti-adiponectin (rat), pAb (Prod. No. AG-25A-0005) at 1:5,000 dilution.
1: Rat serum.
2: Recombinant rat adiponectin.

Specifications / Handling

More Information
Product Details
Synonyms	ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant rat adiponectin.
Application	ELISA: (direct and indirect: 1:5’000-1:10’000) Immunohistochemistry: (frozen sections (1:1’000)) Western Blot: (1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Rat
Specificity	Recognizes rat adiponectin. Weakly cross-reacts with mouse adiponectin. Detects a band of ~35kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

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