AdipoGen Life Sciences

anti-RANKL (human), pAb

CHF 190.00

In stock

AG-25A-0016-C100100 µgCHF 190.00

Figure 1: FACS: RANKL-transfected HEK 293 cells were stained significantly using anti-RANKL (human), pAb (Prod. No. AG-25A-0016).

Figure 2: Western blot analysis using anti-RANKL (human), pAb (Prod. No. AG-25A-0016) at 1:5,000 dilution.
1. Recombinant hRANKL (His-tagged).
2. Human RANKL (GST-tagged).>br /> 3. Con-A activated human T lymphocytes lysate.

Specifications / Handling

More Information
Product Details
Synonyms	TRANCE; ODF; OPGL; TNFSF11; CD254
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human RANKL.
Application	ELISA: (direct and indirect: 1:2'000-1:10'000). Flow Cytometry: (1:500-1:1'000). Western Blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes human RANKL. Detects a band of ~35kDa band by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

RANKL is an osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. Important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Plays an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

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