AdipoGen Life Sciences

anti-RANKL (human), pAb

As low as CHF 190.00

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AG-25A-0016-C100100 µgCHF 190.00

Figure 1: Western blot analysis using anti-RANKL (human), pAb (Prod. No. AG-25A-0016) at 1:5,000 dilution.
1. Recombinant hRANKL (His-tagged).
2. Human RANKL (GST-tagged).>br /> 3. Con-A activated human T lymphocytes lysate.

Figure 2: FACS: RANKL-transfected HEK 293 cells were stained significantly using anti-RANKL (human), pAb (Prod. No. AG-25A-0016).

Specifications / Handling

More Information
Product Details
Synonyms	TRANCE; ODF; OPGL; TNFSF11; CD254
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human RANKL.
Application	ELISA: (direct and indirect: 1:2'000-1:10'000). Flow Cytometry: (1:500-1:1'000). Western Blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes human RANKL. Detects a band of ~35kDa band by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

RANKL is an osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. Important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Plays an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

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