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Figure 1: Western blot analysis using anti-RBP4 (rat), pAb (Biotin) (Prod. No. AG-25A-0039B) at 1:2'000 dilution.
1: Rat RBP4 (His-tagged).
2: Rat RBP4 (FLAG®-tagged).
2: Rat serum (GK/SIc) (1μl).
ELISA: (direct and indirect: 1:2’000-1:5’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity
Rat
Specificity
Recognizes rat RBP4. Detects a band of ~22-25kDa by Western blot.
Purity Detail
Protein A-affinity purified.
Concentration
1mg/ml
Formulation
Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Shipping and Handling
Shipping
BLUE ICE
Short Term Storage
+4°C
Long Term Storage
-20°C
Handling Advice
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability
Stable for at least 6 months after receipt when stored at -20°C.
Retinol binding protein 4 (RBP4; RBP) is a 21kDa secreted protein, a member of the lipocalin family and is known as the primary transporter of retinol (vitamin A) to tissues. A recent report revealed RBP4 as an adipokine linking glucose transporter 4 (GLUT4) suppression in adipose tissue to insulin. Elevated human and mouse serum RBP4 levels are associated with insulin resistance and its severity, obesity, and certain components of metabolic syndrome. Furthermore, human serum RBP4 levels are closely related to renal function.
Product References
Retinol-binding protein 4 and peroxisome proliferator-activated receptor-γ in steatotic liver transplantation: A. Casillas-Ramirez, et al.; J. Pharmacol. Exp. Ther. 338, 143 (2011)