AdipoGen Life Sciences

anti-ANGPTL3 (coiled-coil domain) (human), pAb

CHF 190.00

In stock

AG-25A-0060-C100100 µgCHF 190.00

Figure 1: Immunohistochemical staining of ANGPTL3 using anti-ANGPTL3 (CCD) (human), pAb (Prod. No. AG-25A-0060) in human liver tissue (5µg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Figure 2: Western blot analysis using anti-ANGPTL3 (CCD) (human), pAb (Prod. No. AG-25A-0060) at 1:2'000 dilution.
1: Human ANGPTL3 (CCD) (FLAG^®-tagged).
2: Human ANGPTL3 (FLAG^®-tagged).
3: Human ANGPTL3 (His-tagged).
4: Mouse RBP4 (FLAG^®-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	Angiopoietin-like Protein 3; Angiopoietin-5; ANG-5
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human ANGPTL3.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry: (5μg/ml) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes the coiled-coil domain of human ANGPTL3. Detects a band of ~29kDa by Western blot. Weakly cross-reacts with human full lenght ANGPTL3.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

ANGPTL3 regulates angiogenesis and also directly regulates lipid, glucose, and energy metabolism.

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