AdipoGen Life Sciences

anti-FGF-21 (mouse), pAb

CHF 190.00

In stock

AG-25A-0076-C100100 µgCHF 190.00

Figure 1: Western blot analysis using anti-FGF-21 (mouse), pAb (Prod. No. AG-25A-0076) at 1:4'000 dilution.
1: Mouse FGF-21 (FLAG^®-tagged).
2: Mouse FGF-21 Fc-protein.
3: Human FGF-21 (FLAG^®-tagged).
4: Mouse Nampt (FLAG^®-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	Fibroblast Growth Factor 21
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant mouse FGF-21.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Mouse
Specificity	Recognizes mouse FGF-21. Detects a band of ~25kDa by Western blot. Weakly cross-reacts with human FGF-21.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of bKlotho.

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