anti-FGF-21, mAb (FG224-7)

ELISA: (direct or indirect: 1:2'000-1:5'000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Optimal conditions should be determined individually for each application.

Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.

Recognizes human and mouse FGF-21. Detects a band of ~25kDa by Western blot.

Rat IgG1 Isotype Control