AdipoGen Life Sciences

anti-FGF-21, pAb

CHF 390.00

In stock

AG-25A-0074-C100100 µgCHF 390.00

Figure 1: Western blot analysis of human FGF21 using anti-FGF-21 (human), pAb (Prod. No. AG-25A-0074) at 1:4,000 dilution..
1. Recombinant human FGF21 (FLAG-tagged).
2. Recombinant human FGF21 (Fc protein).
3. Recombinant human FGF21 (His-tagged).
4. Recombinant mouse Visfatin (FLAG-tagged) (negative control).

Figure 2: Immunohistochemical staining of human tissue using anti-FGF-21 (human), pAb (Prod. No. AG-25A-0074) at 1:5000 dilution.
1. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human parathyroid (A), liver (B) and small intestine (C) (100-200x, brown colour).
2. Staining with pre-immune rabbit serum (A-1, B-1, C-1) as negative control (1:500).

Specifications / Handling

More Information
Product Details
Synonyms	Fibroblast Growth Factor 21
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human FGF-21.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry: (paraffin sections 1:2’000-1:5’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human Mouse
Specificity	Recognizes human and mouse FGF-21. Detects a band of ~25kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of bKlotho.

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