AdipoGen Life Sciences

anti-Ribosomal Protein S3 (human), pAb

As low as CHF 140.00

In stock

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AG-25A-0077-TRIAL25 µgCHF 140.00

AG-25A-0077-C100100 µgCHF 280.00

Figure 1: Immunohistochemical staining of RPS3 using using anti-RPS3 (human), pAb (Prod. No. AG-25A-0077) in human colon tissue (5µg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Figure 2: Immunocytochemical staining of RPS3 using using anti-RPS3 (human), pAb (Prod. No. AG-25A-0077) in HT1080 cells (1:100 dilution). Immunofluorescence experiments were performed to determine the intracellular localization of rpS3 in the cytosol and membrane ruffle. (A) x40, (B) x100 objective, respectively.

Figure 3: Western blot and Immunoprecipitation analysis using anti-RPS3 (human), pAb (Prod. No. AG-25A-0077).
Western Blot (1:1'000 dilution):
1: Vector alone-transfected HEK 293 cell lysate (V).
2: RPS3 (FLAG^®-tagged)-transfected HEK 293 cell lysate (FS3).

Immunoprecipitation (1:500 dilution):
3. Anti-mouse IgG.
4. Anti-FLAG.
5. Anti-mouse IgG.
6. Anti-FLAG.
7. Anti-rabbit IgG.
8. Anti-RPS3 pAb.
9. Anti-rabbit IgG.
10. Anti-rabbit RPS3 pAb.

Specifications / Handling

More Information
Product Details
Synonyms	40S Ribosomal Protein S3; IMR-90 Ribosomal Protein S3; RPS3
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human ribosomal protein S3.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunocytochemistry: (1:100) Immunohistochemistry: (5μg/ml) Immunoprecipitation: (1:500-1:5’000) Western Blot: (1:500-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human ribosomal protein S3. Detects a band of ~32kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

RpS3 is a component of the 40S ribosomal subunit and is an an essential but previously unkown subunit of NF-κB involved in the regulation of key genes in rapid cellular activation responses. RpS3 interacts with nm23-H1. The expression of rpS3 reduces the secretion of MMP-9 and the invasive metastatic potential in HT1080 cells. The phosphorylated ERK is reduced by the expression of rpS3.

Product-specific References

Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation: T.S. Kim, et al.; Mol. Biol. Cell 17, 824 (2006)
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK: S.H. Kim & J. Kim; Biochim. Biophys. Acta 1763, 823 (2006)
Arginine methylation of ribosomal protein S3 affects ribosome assembly: H.S. Shin, et al.; BBRC 385, 273 (2009)

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