anti-α-Tubulin, mAb (rec.) (F2C) (ATTO 488)
Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alphaTubulin, mAb (rec.) (F2C) (ATTO 488) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.
|Product Type||Recombinant Antibody|
|Source/Host||Purified from HEK 293 cell culture supernatant.|
|Immunogen/Antigen||Bovine brain tubulin.|
|Specificity||Recognizes mouse, bovine and human α-tubulin.|
|Purity Detail||Protein A-affinity purified.|
|Formulation||Liquid. In PBS containing 0.02% sodium azide.|
|Other Product Data||
anti-α-Tubulin, monoclonal antibody (recombinant) (F2C) (ATTO488) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
anti-α-Tubulin, monoclonal antibody (recombinant) (F2C) (ATTO488) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||+4°C|
Keep conjugated formats at +4°C.
Stable for at least 1 year after receipt when stored at +4°C.
|Product Specification Sheet|
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
- Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003)
- Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al; BMC Biotechnol. 10, 59 (2010)