AdipoGen Life Sciences

anti-Tubulin-GTP, mAb (rec.) (MB11)

CHF 460.00
In stock
AG-27B-0009-C100100 µgCHF 460.00
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Product Details
Product Type Recombinant Antibody
Clone MB11
Isotype Human IgG2λ
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Full length GTP-λ-S loaded tubulin from pig brain.

Immunocytochemistry: (1:10'000)

Crossreactivity Drosophila

Recognizes human, mouse, rat and drosophila tubulin-GTP.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Fc (human) IgG2 Control (rec.)

Other Product Data

anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Product References
  1. Detection of GTP-Tubulin Conformation in Vivo Reveals a Role for GTP Remnants in Microtubule Rescues: A. Dimitrov, et al.; Science 322, 1353 (2008)
  2. Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport: T. Nakata, et al.; J. Cell Biol. 194, 245 (2011)
  3. Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the Presynapse: P. Guedes-Dias, et al.; Curr. Biol. 29, 268 (2019)
  4. Molecular understanding of label-free second harmonic imaging of microtubules: V. Van Steenbergen, et al.; Nat. Commun. 10, 3530 (2019)
  5. Golgi‐associated microtubules are fast cargo tracks and required for persistent cell migration: H. Hao, et al.; EMBO Rep. 21, e48385 (2020)
  6. Tubulin binding potentially clears up Bortezomib and Carfilzomib differential neurotoxic effect: A. Malacrida, et al.; Nature Sci. Rep. 11, 10523 (2021)
  7. Motor usage imprints microtubule stability along the shaft: M. Andreu-Carbó, et al.; Dev. Cell 57, 5 (2022)
  8. Phosphomimetic Mutation at Ser165 of α‑Tubulin Promotes the Persistence of GTP Caps in Microtubules: V. Maddula, et al.; Biochem. 61, 1508 (2022)
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