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AdipoGen Life Sciences
anti-β-Tubulin, mAb (rec.) (S11B)
360
CHF
CHF 360.00
In stock
AG-27B-0008-C100100 µgCHF 360.00
Figure 1: Schematic antibody structure.
Figure 2: Human β-Tubulin is detected by immunocytochemistry using anti-β-Tubulin, mAb (rec.) (S11B) (Prod. No. AG-27B-0008).
Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-beta-Tubulin, mAb (rec.)(S11B)(1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-beta-Tubulin, mAb (rec.)(S11B)(1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
| Product Details | |
|---|---|
| Synonyms | Tubulin β Chain, Tubulin β-5 chain |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | S11B |
| Isotype | Human IgG2λ |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Full length tubulin from pig brain. |
| Application |
ELISA |
| Crossreactivity |
Drosophila Human Monkey Mouse Pig Rat |
| Specificity |
Recognizes human, mouse, rat, pig, drosophila and monkey β-tubulin. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
anti-β-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability |
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
anti-β-Tubulin, monoclonal antibody (recombinant) (S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Product References
- Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003)
- WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity: E. Steib, et al.; eLife 9, e57205 (2020)
- Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM): M.H. Laporte, et al.; Nat. Methods 19, 216 (2022)
- CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity: M.D. Arslanhan, et al.; J. Cell Biol. 222, e202305009 (2023)
- Protofilament-specific nanopatterns of tubulin post-translational modifications regulate the mechanics of ciliary beating: G. Alvarez Viar, et al.; Curr. Biol. 34, 4464 (2024)
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