AdipoGen Life Sciences

anti-Rab1-GTP, mAb (rec.) (ROF7)

CHF 390.00
In stock
AG-27B-0006-C100100 µgCHF 390.00
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Product Details
Synonyms Ras-related Protein Rab-1
Product Type Recombinant Antibody
Properties
Clone ROF7
Isotype Human IgG2λ
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Full length canine Rab1.
Application

Immunocytochemistry: (1:1000)
Immunoprecipitation: (1:200)

Crossreactivity Dog
Human
Mouse
Rat
Specificity

Recognizes human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Fc (human) IgG2 Control (rec.)

Other Product Data

anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking.

anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Product References
  1. Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al.; BMC Biotechnol. 10, 59 (2010)
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