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AdipoGen Life Sciences
anti-c-Myc, mAb (rec.) (9E10 improved variant) (Fc Mouse)
330
CHF
CHF 330.00
In stock
AG-27B-6302-C05050 µgCHF 330.00
Figure 1: Quantitative immunodetection of a myc tagged protein using the automated Protein Simple™ Western Immunoassay system. Quantified chemiluminescence was automatically converted to gel-like pictures by the system. a, whole chromatogram, b, comparison of the detected bands, either stained with 9E10 or anti-c-Myc, mAb (rec.) (9E10 improved variant). anti-c-Myc, mAb (rec.) (9E10 improved variant) provides improved detection sensitivity compared to hybridoma antibody 9E10.
Figure 2: Improvement of affinitiy and Fv-region thermal stability of anti-c-Myc, mAb (rec.) (9E10 improved variant) compared to hybridoma antibody Myc1-9E10 recognizing the myc-epitope.
Figure 3: Whole mount of 2 dpf transgenic zebrafish expressing the myc-epitope as a fusion to a fluorescent protein (mblbp-E1b: mScarlet-myc) stained with anti-c-Myc, mAb (rec.) (9E10 improved variant) and fluorescently labelled secondary anti-mouse Fc antibody.
| Product Details | |
|---|---|
| Synonyms | HyperMyc; c-Myc-tag; Myc Proto-oncogene Protein; Class E Basic Helix-loop-helix Protein 39; HLHe39; Transcription Factor p64 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | TUN219-2C1 (Hypermyc) – 9E10 improved variant |
| Isotype | Mouse IgG2a |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Synthetic peptide corresponding to aa408-439 of human c-Myc. |
| Application |
ELISA |
| Specificity |
Recognizes the c-Myc tag. Shows no binding to native c-Myc protein. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from animal component-free supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
This is an antibody developed by antibody phage display technology using a human naive antibody gene library. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies. It has a Y-shaped structure of a common full-length IgG, consisting of 4 polypeptide, 2 heavy-chains and 2 light-chains. |
| Accession Number | P01106 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 3 months after receipt when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti-c-Myc Tag antibody. It showed a higher affinity and stability in direct comparison to hybridoma antibody 9E10 (anti-c-myc-tag) that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity [Reference 2]. This antibody can be detected with anti-mouse Fc secondary antibodies.
Product References
- A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations: F. Bertoglio, et al.; Cell Rep. 36, 109433 (2021)
- In vitro evolution of myc-tag antibodies: in-depth specificity and affinity analysis of Myc1-9E10 and Hyper-Myc: G. Russo, et al.; Biol. Chem. 403, 479 (2022)
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