Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
anti-c-Myc, mAb (rec.) (9E10 improved variant) (Fc Rabbit)
502
CHF
CHF 502.00
In stock
AG-27B-6303-C05050 µgCHF 502.00
Figure 1: Quantitative immunodetection of a myc tagged protein using the automated Protein Simple™ Western Immunoassay system. Quantified chemiluminescence was automatically converted to gel-like pictures by the system. a, whole chromatogram, b, comparison of the detected bands, either stained with 9E10 or anti-c-Myc, mAb (rec.) (9E10 improved variant). anti-c-Myc, mAb (rec.) (9E10 improved variant) provides improved detection sensitivity compared to hybridoma antibody 9E10.
Figure 2: Improvement of affinitiy and Fv-region thermal stability of anti-c-Myc, mAb (rec.) (9E10 improved variant) compared to hybridoma antibody Myc1-9E10 recognizing the myc-epitope.
Figure 3: Whole mount of 2 dpf transgenic zebrafish expressing the myc-epitope as a fusion to a fluorescent protein (mblbp-E1b: mScarlet-myc) stained with anti-c-Myc, mAb (rec.) (9E10 improved variant) and fluorescently labelled secondary anti-mouse Fc antibody.
| Product Details | |
|---|---|
| Synonyms | HyperMyc; c-Myc-tag; Myc Proto-oncogene Protein; Class E Basic Helix-loop-helix Protein 39; HLHe39; Transcription Factor p64 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | TUN219-2C1 (Hypermyc) – 9E10 improved variant |
| Isotype | Rabbit IgG |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Synthetic peptide corresponding to aa408-439 of human c-Myc. |
| Application |
ELISA |
| Specificity |
Recognizes the c-Myc tag. Shows no binding to native c-Myc protein. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from animal component-free supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
This is an antibody developed by antibody phage display technology using a human naive antibody gene library. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies. It has a Y-shaped structure of a common full-length IgG, consisting of 4 polypeptide, 2 heavy-chains and 2 light-chains. |
| Accession Number | P01106 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 3 months after receipt when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The c-Myc or myc-tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti-c-myc tag antibody. It showed a higher affinity and stability in direct comparison to hybridoma antibody 9E10 (anti-c-myc-tag). This antibody can be detected with anti-rabbit Fc secondary antibodies.
Product References
- A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations: F. Bertoglio, et al.; Cell Rep. 36, 109433 (2021)
- In vitro evolution of myc-tag antibodies: in-depth specificity and affinity analysis of Myc1-9E10 and Hyper-Myc: G. Russo, et al.; Biol. Chem. 403, 479 (2022)
Related Products
