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Figure 1: ELISA binding assay with S1 and RBD Protein of different corona strain mutants (produced in Hi5 cells with His-tag) using anti-SARS-CoV-2 Spike Protein S1 (RBD), mAb (rec.) (AB68-A09) (Fc Human) (#AG-27B-6310).
Figure 2: ELISA binding assay with S1 Protein of different beta-corona strains using anti-SARS-CoV-2 Spike Protein S1 (RBD), mAb (rec.) (AB68-A09) (Fc Human) (AG-27B-6310).
Figure 3: ELISA binding assay with S1 Protein of different corona strain mutants (produced in Hi5 cells) using anti-SARS-CoV-2 Spike Protein S1 (RBD), mAb (rec.) (AB68-A09) (Fc Human) (AG-27B-6310).
Figure 4: Sandwich ELISA assay with S1 protein of SARS-Cov-2. anti-SARS-CoV-2 Spike Protein S1 (RBD), mAb (rec.) (AB68-A09) (Fc Human) (AG-27B-6310) was coated into MTP wells for antigen capture. Recombinant S1 (His-tagged) was titered starting from a concentration of 31.6 nM to 0.03 nM. Antibody-captured S1 was detected using anti-SARS-CoV-2 Spike Protein S1 (NTD), mAb (rec.) (AB72-1-G09) (AG-27B-6307) followed by anti-mouse-HRP staining.
Figure 5: Calu-3 cells were infected with SARS-CoV-2 (B.1.1.7 or Zagreb), fixed with formaldehyde and stained with anti-SARS-CoV-2 Spike Protein S1 (RBD), mAb (rec.) (AB68-A09) (Fc Human) (AG-27B-6310) followed by anti-human-Alexa488 staining and a nuclear marker.
2019-nCoV Spike Protein S1 (RBD); Spike Receptor Binding Domain
Product Type
Recombinant Antibody
Properties
Clone
AB68-A09
Isotype
Human IgG1
Source/Host
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen
Recombinant SARS-CoV-2 S1 receptor binding protein (aa 319-591) containing a C-terminal human Fc-tag.
Application
ELISA Immunocytochemistry Western Blot (non-reducing)
Crossreactivity
Virus
Specificity
Recognizes the SARS-CoV-2 S1 (Receptor-binding domain) of different mutant variants, including Omicron. Does not cross-react with HCoV-OC43, HCoV-229E, HCoV-NL63, HCoV-HKU1, MERS-CoV or SARS-CoV.
Purity
≥95% (SDS-PAGE)
Purity Detail
Protein A purified from animal component-free supernatant.
This is an antibody developed by antibody phage display technology using a human naive antibody gene library. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies. It has a Y-shaped structure of a common full-length IgG, consisting of 4 polypeptide, 2 heavy-chains and 2 light-chains.
Accession Number
P0DTC2 (aa 319-541)
Declaration
Manufactured by Abcalis
Shipping and Handling
Shipping
BLUE ICE
Short Term Storage
+4°C
Long Term Storage
-20°C
Handling Advice
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination.
Use/Stability
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 3 months after receipt when stored at +4°C.
Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S protein plays a key role in viral infection and pathogenesis. The S1 subunit contains the N-terminal domain (NTD) and a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells, whereas S2 harbors heptad repeat 1 (HR1) and HR2. The RBD domain first binds its receptor to form an RBD/ACE2 complex. This triggers conformational changes in the S protein, leading to membrane fusion mediated via HR1 and HR2 and consequently in viral entry into target cells. Antibodies targeting various regions of S protein have different mechanisms in inhibiting SARS-CoV-2 infection. For example, NTD-targeting antibodies bind the NTD to form an NTD/mAb complex, thereby preventing conformational changes in the S protein and blocking membrane fusion and viral entry. RBD-targeting antibodies form RBD/mAb or RBD/Nb complexes that inhibit binding of the RBD to ACE2, thereby preventing entry of SARS-CoV-2 into target cells.
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