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AdipoGen Life Sciences
anti-SARS-CoV-2 N Protein, mAb (rec.) (AB79-E11) (Fc Mouse)
330
CHF
CHF 330.00
In stock
AG-27B-6305-C05050 µgCHF 330.00
Figure 1: anti-SARS-CoV-2 N Protein, mAb (rec.) (AB79-E11)(AG-27B-6305) was coated into MTP wells for antigen capture. Triton X-inactivated virion particles (fzmb) were titered starting from a concentration of 20´000 PFU/mL down to 20 PFU/mL. Antibody-captured virion particles were detected using human polyclonal anti-SARS-CoV-2 antibody.
Figure 2: anti-SARS-CoV-2 N Protein, mAb (rec.) (AG-27B-6305) was coated into MTP wells for antigen capture. Recombinant N-protein (His-tagged) was titered starting from a concentration of 31.6 nM. Antibody-captured N-protein was detected using human polyclonal anti-SARS-CoV-2 antibody.
Figure 3: ELISA binding assay with nucleocapsid protein (N-protein) of different beta-corona strains using anti-SARS-CoV-2 N Protein, mAb (rec.) (AG-27B-6305).
Figure 4: Sandwich ELISA assay with nucleocapsid protein of SARS-CoV-2 (N-protein). anti-SARS-CoV-2 N Protein, mAb (rec.) (AG-27B-6305) was coated into MTP wells for antigen capture. Recombinant N-protein (His-tagged) was titered starting from a concentration of 12.6 nM to 0.01 nM. Antibody-captured N-protein was detected using anti-SARS-CoV-2 N Protein, mAb (rec.) (AB75-C03) (AG-27B-6304) followed by anti-mouse-HRP staining.
Figure 5: Calu-3 cells were infected with SARS-CoV-2, fixed with formaldehyde and stained with anti-SARS-CoV-2 N Protein, mAb (rec.) (AG-27B-6305) followed by anti-mouse-Alexa488 staining and a nuclear marker.
| Product Details | |
|---|---|
| Synonyms | 2019-nCoV N Protein; Nucleoprotein; Nucleocapsid Protein |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | AB79-E11 |
| Isotype | Mouse IgG2a |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Recombinant SARS-CoV-2 Nucleocapsid protein (aa 1-419) containing a C-terminal His-tag. |
| Application |
ELISA |
| Crossreactivity | Virus |
| Specificity |
Recognizes the SARS-CoV-2 Nucleocapsid protein. Shows cross-reactivity to SARS-CoV-1 N-protein. Does not cross-react with HCoV-OC43, HCoV-229E, HCoV-NL63, HCoV-HKU1 or MERS-CoV. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from animal component-free supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
This is an antibody developed by antibody phage display technology using a human naive antibody gene library. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies. It has a Y-shaped structure of a common full-length IgG, consisting of 4 polypeptide, 2 heavy-chains and 2 light-chains. |
| Accession Number | P59595 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 3 months after receipt when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19).SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms. The N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M.
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