AdipoGen Life Sciences

anti-SARS-CoV-2 N Protein, mAb (rec.) (AB75-C03)

CHF 330.00
In stock
AG-27B-6304-C05050 µgCHF 330.00
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Product Details
Synonyms 2019-nCoV N Protein; Nucleoprotein; Nucleocapsid Protein
Product Type Recombinant Antibody
Properties
Clone AB75-C03
Isotype Mouse IgG2a
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Recombinant SARS-CoV-2 Nucleocapsid protein (aa 1-419) containing a C-terminal His-tag.
Application

ELISA
Immunocytochemistry
Western Blot
Tested against different coronaviruses and against different preparations of inactivated SARS-CoV-2 virion particles.

Crossreactivity Virus
Specificity

Recognizes the SARS-CoV-2 Nucleocapsid protein. Shows cross-reactivity to SARS-CoV-1 N-protein. Does not cross-react with HCoV-OC43, HCoV-229E, HCoV-NL63, HCoV-HKU1 or MERS-CoV.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A purified from animal component-free supernatant.
Concentration 1 mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Mouse IgG2a Isotype Control

Other Product Data

This is an antibody developed by antibody phage display technology using a human naive antibody gene library. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies. It has a Y-shaped structure of a common full-length IgG, consisting of 4 polypeptide, 2 heavy-chains and 2 light-chains.

Accession Number P59595
Declaration Manufactured by Abcalis
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Please handle under sterile conditions to avoid contamination.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Stable for at least 3 months after receipt when stored at +4°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19).SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms. The N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M.

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