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AdipoGen Life Sciences
Resistin (human) (rec.)
265
CHF
CHF 265.00
In stock
AG-40A-0010Y-C01010 µgCHF 265.00
AG-40A-0010Y-C05050 µgCHF 780.00
Figure 1: Effect of Resistin (human) (rec.) (AG-40A-0010Y) on glucose uptake in L6 cells. Inhibition of insulin-stimulated glucose uptake in L6 skeletal muscle cells by resistin (as expected: 1μg/ml). Rat myoblasts (L6) were cultured and differentiated into myotubes. Differentiated L6 cells were exposed to resistin for various time and then incubated with or without insulin.
| Product Details | |
|---|---|
| Synonyms | FIZZ3; Found in Inflammatory Zone 3; ADSF; Adipose Tissue-Specific Secretory Factor |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | Human resistin (aa 19-108) is fused at the N-terminus to a FLAG®-tag. |
| Crossreactivity | Human |
| Biological Activity | Inhibits insulin-stimulated glucose uptake in L6 skeletal muscle cells. |
| MW | ~13kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 50µg size: 1 mg/ml |
| Reconstitution |
10µg size: Reconstitute with 100µl sterile water. 50µg size: Reconstitute with 50µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
UniProt link Q9HD89: Resistin (human) (precursor) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELM-α/FIZZ 1 and RELM-β/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.
Product References
- Expression of resistin in the prostate and its stimulatory effect on prostate cancer cell proliferation: H.J. Kim, et al.; BJU Int. 108: 1464 (2010)
- Aptamer duo-based portable electrochemical biosensors for early diagnosis of periodontal disease: C. Joe, et al.; Biosensors Bioelectronics 199, 113884 (2022)
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