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AdipoGen Life Sciences
Progranulin (rat) (rec.)
190
CHF
CHF 190.00
In stock
AG-40A-0194-C01010 µgCHF 190.00
AG-40A-0194-C05050 µgCHF 390.00
Figure 1: Deglycosylation of rat progranulin. To examine the deglycosylation of rat Progranulin-FLAG, 1 μg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100°C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37°C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.
Figure 2: NH2-terminal sequence analysis.
| Product Details | |
|---|---|
| Synonyms | Proepithelin; PEPI; PC Cell-derived Growth Factor |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Signal peptide and rat progranulin (aa 1-602) are fused at the C-terminus to a FLAG®-tag. |
| Crossreactivity | Rat |
| MW | ~90kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
| Other Product Data |
Genome Net link NP_058809: Progranulin (rat) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer‘s Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis. EphA2, a member of the large family of Ephrin receptor tyrosine kinases, is also a functional signaling receptor for progranulin as reported recently.
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