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AdipoGen Life Sciences
RANKL, Soluble (human) (rec.)
As low as
205
CHF
CHF 205.00
In stock
Only %1 left
AG-40B-0008-C01010 µgCHF 205.00
AG-40B-0008-30103 x 10 µgCHF 410.00
Figure 1: ELISA binding assay between human RANKL (Prod. No. AG-40B-0008) and mouse RANK:Fc (Prod. No. AG-40B-0092). The EC50 =15ng/ml on the mouse RANK receptor. Not shown, the EC50=1ng/ml on the human RNK receptor.
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | TRANCE; ODF; OPGL; TNFSF11; CD254 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | Human RANKL (aa 152-317) is fused at the N-terminus to a FLAG®-tag. |
| Crossreactivity |
Human Mouse |
| Specificity | Binds to human and mouse RANK. |
| MW | ~28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data | UniProt link O14788: RANKL (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
RANKL is an osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T cell proliferation. Important regulator of interactions between T cells and dendritic cells and may play a role in the regulation of the T cell-dependent immune response. RANKL plays an important role in the progression of breast cancer.
Product-specific References
- Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001)
- Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001)
- Suppression of IL-12 Production by Soluble CD40 Ligand: Evidence for Involvement of the p44/42 Mitogen-Activated Protein Kinase Pathway: M. Wittmann, et al.; J. Immunol. 168, 3793 (2002)
- Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004)
- Osteoprotegerin production by human intestinal epithelial cells: a potential regulator of mucosal immune responses: K. Vidal, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 287, G836 (2004)
- A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004)
- Mechanisms of spontaneous osteoclastogenesis in cancer with bone involvement: I. Roato, et al.; FASEB J. 19, 228 (2005)
- Icariin inhibits the osteoclast formation induced by RANKL and macrophage-colony stimulating factor in mouse bone marrow culture: K.M. Chen, et al.; Pharmazie 62, 388 (2007)
- Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL) Modulates the Expression of Genes Involved in Apoptosis and Cell Cycle in Human Osteoclasts: E. Rimondi, et al.; Anatom. Rec. 290, 838 (2007)
- Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective: W.-L. Wang, et al.; Biomed. Res. Int. 2014, 853234 (2014
- Tumor necrosis factor receptor family costimulation increases regulatory T-cell activation and function via NF-κB: M. Lubrano di Ricco, et al.; Eur. J. Immunol. 50, 972 (2020)
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