TNF-α (human) (multimeric) (rec.)
|Synonyms||MultimericTNF-α™; ACRP30headless:TNF-α; ACRP30headless:TNFSF2; ACRP30headless:Tumor Necrosis Factor-α|
|Source/Host||HEK 293 cells|
|Sequence||The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.|
|Specificity||Binds to human and mouse TNF-R1 and TNF-R2.|
|Biological Activity||Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).|
|Endotoxin Content||<0.02EU/μg purified protein (LAL test; Lonza).|
|Concentration||0.1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 100μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||
UniProt link P01375: TNF-α (human)
FLAG is a registered trademark of Sigma-Aldrich Co.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-α™ is a high activity construct in which two trimeric TNF-α ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (TNF-α (human) (multimeric) (rec.) (Prod. No. AG-40B-0019). Since the MultimericTNF-α™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or MultimericTNF-α™ can be used.
- TNFR2 induced priming of the inflammasome leads to a RIPK1-dependent cell death in the absence of XIAP: J. Knop, et al.; Cell Death Dis. 10, 700 (2019)
- CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity: J. Joung, et al.; Nature Commun. 13, 1606 (2022)