TNF-α (mouse) (multimeric) (rec.)

CHF 230.00
In stock
AG-40B-0021-C01010 µgCHF 230.00
AG-40B-0021-30103 x 10 µgCHF 460.00
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Product Details
Synonyms MultimericTNF-α™; ACRP30headless:TNF-α; ACRP30headless:TNFSF2; ACRP30headless:Tumor Necrosis Factor-α
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence The extracellular domain of mouse TNF-α (aa 77-235) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
Crossreactivity Human
Mouse
Specificity Binds to human and mouse TNF-R1 and TNF-R2.
Biological Activity Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
MW ~34kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.02EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link P06804: TNF-α (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-α™ is a high activity construct in which two trimeric TNF-α ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (TNF-α (mouse) (multimeric) (rec.) (Prod. No. AG-40B-0021). Since the MultimericTNF-α™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or MultimericTNF-α™ can be used.

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