FasL (human) (multimeric) (rec.)

CHF 420.00
In stock
AG-40B-0130-C01010 µgCHF 420.00
AG-40B-0130-30103 x 10 µgCHF 840.00
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Product Details
Synonyms MultimericFasL™; MegaFasL; ACRP30headless:APO-1L; ACRP30headless:CD95L; ACRP30headless:CD178; ACRP30headless:TNFSF6; ACRP30headless:Fas Ligand
Product Type Protein
Source/Host HEK 293 cells

Human FasL (aa 139-281) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Crossreactivity Human

Binds to human and mouse Fas.

Biological Activity

Induces apoptosis of human Jurkat T cells at a concentration of <0.2ng/ml.

MW ~40kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.02EU/μg purified protein (LAL test).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link P48023: FasL (human)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

MultimericFasL™ is a high activity construct in which two trimeric FasL are artificially linked via the collagen domain of ACRP30. This construct very effectively mimics the natural membrane-assisted aggregation of FasL in vivo. FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. It is involved in cytotoxic T cell mediated apoptosis and in T cell development. 

FasL treatment of stem cells is a robust method for proliferating cells in culture while improving their stemness properties. Treatment with FasL results in a large quantity of Adipose Derived Stem Cells (ASC) of high quality.

Product References
  1. Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003)
  2. A Fas agonist induces high levels of apoptosis in haematological malignancies: P. Greaney, et al.; Leuk. Res. 30, 415 (2006)
  3. Apograft, a Novel Stem Cell Selection Technology, Prevents Graft vs. Host Disease (GvHD) While Preserving Graft vs Leukemia (GvL) Effects: J. Stein, et al.; Biol. Blood Marrow Transplant. 24, 192 (2018)
  4. ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages: B.L. Lee, et al.; Sci. Rep. 8, 3788 (2018)
  5. Hypertonicity-enforced BCL-2 addiction unleashes the cytotoxic potential of death receptors: S. Sirtl, et al.; Oncogene 37, 4122 (2018)
  6. Fas Promotes T Helper 17 Cell Differentiation and Inhibits T Helper 1 Cell Development by Binding and Sequestering Transcription Factor STAT1: G. Meyer zu Horste, et al.; Immunity 48, 556 (2018)
  7. Fas-L promotes the stem cell potency of adipose-derived mesenchymal cells: I. Solodeev, et al.; Cell Death Dis. 9, 695 (2018)
  8. IRF2 transcriptionally induces GSDMD expression for pyroptosis: N. Kayagaki, et al.; Sci. Signal. 12, eaax4917 (2019)
  9. Brief ex vivo Fas-ligand incubation attenuates GvHD without compromising stem cell graft performance: H. Levy-Barazany, et al.; Bone Marrow Transplant. 55, 1305 (2020)
  10. NINJ1 mediates plasma membrane rupture during lytic cell death: N. Kayagaki, et al.; Nature 591, 131 (2021)
  11. FasL Promotes Proliferation and Delays Differentiation of ASCs: I. Sarel, et al.; Jpn. J. Med. 4, 474 (2021)
  12. Fas-threshold signalling in MSCs promotes pancreatic cancer progression and metastasis: A. Mohr, et al.; Cancer Lett. 519, 63 (2021)
  13. Smac mimetics and TRAIL cooperate to induce MLKL-dependent necroptosis in Burkitt’s lymphoma cell lines: A. Koch, et al. Neoplasia 23, 539 (2021)
  14. Immuno-suppressive hydrogels enhance allogeneic MSC survival after transplantation in the injured brain: M. Alvarado-Velez, et al.; Biomaterials 266, 120419 (2021)
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