AdipoGen Life Sciences

Fc (human):FasL, Soluble (human) (rec.)

CHF 335.00

In stock

AG-40B-0132-C01010 µgCHF 335.00

AG-40B-0132-30103 x 10 µgCHF 670.00

Figure 1: Oligomerisation of FasL (h) efficiently triggers Jurkat cell death.
Method: Jurkat cells were treated O/N with the indicated concentrations of MegaFasL, Soluble (human) (rec.) (Prod. No. AG-40B-0130), Fc:FasL, Soluble (human) (rec.) (Prod. No. AG-40B-0132), FasL, Soluble (human) (rec.) (Prod. No. AG-40B-0001) or FasL, Soluble (human) (rec.) + TNF ligands enhancer (Prod. No. AG-44B-0001) (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (MegaFasL, Soluble (human), Fc:FasL, Soluble (human) and FasL, Soluble (human) + TNF ligands enhancer) kill Jurkat cells at IC₅₀ <0.2ng/ml.

Specifications / Handling

More Information
Product Details
Synonyms	APO-1L; CD95L; CD178; TNFSF6; Fas Ligand
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	Human FasL (aa 139-281) is fused at the N-terminus to the Fc portion of human IgG1.
Crossreactivity	Human Mouse
Specificity	Binds to human and mouse Fas.
Biological Activity	Induces apoptosis of human Jurkat T cells at a concentration of <0.2ng/ml.
MW	~50kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg purified protein (LAL test).
Concentration	0.1mg/ml after reconstitution.
Reconstitution	Reconstitute with 100μl sterile water.
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.)
Other Product Data	UniProt link P48023: FasL (human) FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Fc (human):FasL, Soluble (human) is a high activity construct in which two trimeric FasL are artificially linked via the Fc binding domain of human IgG1. This construct very effectively simulates the natural membrane-assisted aggregation of FasL in vivo. FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. It is involved in cytotoxic T cell mediated apoptosis and in T cell development.

Product References

Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003)
Immune response to intravenous immunoglobulin in patients with Kawasaki disease and MIS-C: Y.P. Zhu, et al.; J. Clin. Invest. 131, e147076 (2021)
NET formation is a default epigenetic program controlled by PAD4 in apoptotic neutrophils: Y.P. Zhu, et al.; Sci. Adv. 9, 1397 (2023)

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