AdipoGen Life Sciences

FasL, Soluble (human) (rec.)

CHF 190.00

In stock

AG-40B-0001-C01010 µgCHF 190.00

AG-40B-0001-30103 x 10 µgCHF 385.00

More Information
Product Details
Synonyms	APO-1L; CD95L; CD178; TNFSF6; Fas Ligand
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	The extracellular domain of human FasL (aa 103-281) is fused at the N-terminus to a FLAG^®-tag.
Crossreactivity	Human Mouse
Biological Activity	Induces apoptosis of human Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1 to 1μg/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, FasL is working at 50-100 fold higher concentrations.
MW	~33kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.05EU/μg purified protein (LAL test; Lonza).
Concentration	0.1mg/ml after reconstitution.
Reconstitution	Reconstitute with 100μl sterile water.
Formulation	Lyophilized. Contains PBS.
Other Product Data	UniProt link P48023: FasL (human) FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. Is involved in cytotoxic T cell mediated apoptosis and in T cell development.

Product References

Tumor necrosis factor receptor family costimulation increases regulatory T-cell activation and function via NF-κB: M. Lubrano di Ricco, et al.; Eur. J. Immunol. 50, 972 (2020)
Targeting adrenergic receptors to mitigate invariant natural killer T cells-induced acute liver injury: MB. Gonzatti, et al.; iScience 26, 107947 (2023)

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