AdipoGen Life Sciences

TNF-α (human) (multimeric) (rec.)

As low as CHF 215.00

In stock

Only %1 left

AG-40B-0019-C01010 µgCHF 215.00

AG-40B-0019-30103 x 10 µgCHF 430.00

Equivalent to MegaLigands

Specifications / Handling

More Information
Product Details
Synonyms	MultimericTNF-α™; ACRP30headless:TNF-α; ACRP30headless:TNFSF2; ACRP30headless:Tumor Necrosis Factor-α
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG^®-tag.
Crossreactivity	Human Mouse
Specificity	Binds to human and mouse TNF-R1 and TNF-R2.
Biological Activity	Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED₅₀= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
MW	~34kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.02EU/μg purified protein (LAL test; Lonza).
Concentration	0.1mg/ml after reconstitution.
Reconstitution	Reconstitute with 100μl sterile water.
Formulation	Lyophilized. Contains PBS.
Other Product Data	UniProt link P01375: TNF-α (human) FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-α™ is a high activity construct in which two trimeric TNF-α ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (TNF-α (human) (multimeric) (rec.) (Prod. No. AG-40B-0019). Since the MultimericTNF-α™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or MultimericTNF-α™ can be used.

Product-specific References

TNFR2 induced priming of the inflammasome leads to a RIPK1-dependent cell death in the absence of XIAP: J. Knop, et al.; Cell Death Dis. 10, 700 (2019)
CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity: J. Joung, et al.; Nature Commun. 13, 1606 (2022)

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