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AdipoGen Life Sciences
TNF-α (human) (multimeric) (rec.)
215
CHF
CHF 215.00
In stock
AG-40B-0019-C01010 µgCHF 215.00
AG-40B-0019-30103 x 10 µgCHF 430.00
| Product Details | |
|---|---|
| Synonyms | MultimericTNF-α™; ACRP30headless:TNF-α; ACRP30headless:TNFSF2; ACRP30headless:Tumor Necrosis Factor-α |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
| Crossreactivity |
Human Mouse |
| Specificity | Binds to human and mouse TNF-R1 and TNF-R2. |
| Biological Activity | Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells). |
| MW | ~34kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.02EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
UniProt link P01375: TNF-α (human) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-α™ is a high activity construct in which two trimeric TNF-α ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (TNF-α (human) (multimeric) (rec.) (Prod. No. AG-40B-0019). Since the MultimericTNF-α™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or MultimericTNF-α™ can be used.
Product References
- TNFR2 induced priming of the inflammasome leads to a RIPK1-dependent cell death in the absence of XIAP: J. Knop, et al.; Cell Death Dis. 10, 700 (2019)
- CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity: J. Joung, et al.; Nature Commun. 13, 1606 (2022)
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