OX40L (mouse) (multimeric) (rec.)
|Synonyms||MultimericOX40L™; ACRP30headless:OX40L; ACRP30headless:CD252; ACRP30headless:CD134L|
|Source/Host||HEK 293 cells|
|Sequence||The extracellular domain of mouse OX40L (aa 52-199) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.|
|Specificity||Binds to human and mouse OX40 [CD134].|
|Biological Activity||Activates T cell proliferation.|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Concentration||0.1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 100μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||
UniProt link P43488: OX40L (mouse)
FLAG is a registered trademark of Sigma-Aldrich Co.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
OX40L [CD252; CD134L] is a membrane-expressed cytokine that belongs to the TNF ligand family. It acts as costimulator through interaction with its receptor OX40 on T cells, stimulating T cell activation, proliferation and cytokine production. OX40L is expressed on antigen presenting cells including B cells, dendritic cells, mast cells and endothelium. MultimericOX40L is a high activity construct in which two trimeric OX40L ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively activates T cell proliferation.
- Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA: J. Mestas, et al.; Int. Immun. 17, 737 (2005)