AdipoGen Life Sciences

IL-36Ra (aa 3-156) (mouse) (rec.) (untagged)

CHF 190.00
In stock
AG-40B-0096-C01010 µgCHF 190.00
AG-40B-0096-30103 x 10 µgCHF 385.00
Replaces AG-40B-0057
More Information
Product Details
Synonyms Interleukin-36 Receptor Antagonist Protein; IL-1F5; Interleukin-1 Family Member 5; IL-1δ; Interleukin-1δ; Interleukin-1 Homolog 3; Interleukin-1-like Protein 1
Product Type Protein
Properties
Source/Host E. coli
Sequence Mouse IL36Ra (aa Val3-Asp156) is untagged.
Crossreactivity Mouse
Application Inhibition of biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9).
Biological Activity Inhibits the IL-6 induced expression of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9) in BMDCs. 1μg/ml of IL-36Ra inhibits the activity of 10 ng to 100 ng/ml of the agonists.
MW ~17kDa
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.05EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data Uni-Prot link Q9QYY1: Interleukin-36 receptor antagonist protein
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

IL-36Ra/IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to IL-36α (IL-1F6), IL-36β (IL-1F8) and IL-36γ (IL-1F9). These cytokines bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1β, IL-6, TNF-α and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are target of the IL-36 interleukins leading to a Th1 response. Recently mutations that affect the levels and the activity of IL-36Ra have been found in patients with pustular psoriasis, leading to enhanced production of inflammatory cytokines (IL-8 in particular) by keratinocytes.

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