AdipoGen Life Sciences

IL-36β (aa 31-183) (mouse) (rec.) (untagged)

CHF 230.00
In stock
AG-40B-0099-C01010 µgCHF 230.00
More Information
Product Details
Synonyms Interleukin-36β; Interleukin-1 Family Member 8; IL-1F8
Product Type Protein
Properties
Source/Host E. coli
Sequence Mouse IL-36β (aa Ser31-Lys183) is untagged.
Crossreactivity Mouse
Biological Activity Induces secretion of IL-6 in BMDCs at a concentration of 10 - 100ng/ml.
MW ~17kDa
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS + 0.5% Trehalose.
Other Product Data Uni-Prot link Q9D6Z6: Interleukin-36 β
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

IL-36α (IL-1F6), IL-36β (IL-1F8) and IL-36γ (IL-1F9) bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1 and are inhibited by IL-36Ra. The expression of IL-36 cytokines has been shown to occur at different sites including the lung and skin and can be derived from diverse cell types including keratinocytes, bronchial epithelium as well as macrophages, monocytes and different T cell subsets. IL-36 family members induce the production of proinflammatory cytokines, including IL-12, IL-1β, IL-6, TNF-α and IL-23 in BMDC and CD4 T cells. Skin and dendritic cells are targets of the IL-36 interleukins leading to a Th1 response. These cytokines may represent potential targets for immune-mediated inflammatory conditions or, alternatively, could be used as adjuvants in vaccination.

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