Angiopoietin-2 (human) (recombinant protein)

Specifications / Handling

More Information
Product Details
Synonyms	Ang-2; Ang2; Angpt2
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	Human angiopoietin-2 (aa 19-496) is fused at the N-terminus to a FLAG^®-tag.
Crossreactivity	Human Mouse
Specificity	Binds to the human and mouse Tie-2 receptor.
MW	~70kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg purified protein (LAL test; Lonza).
Concentration	0.1 mg /ml after reconstitution.
Reconstitution	Reconstitute with 100μl sterile water.
Formulation	Lyophilized. Contains PBS with trehalose and Brij35.
Other Product Data	UniProt link O15123: Angiopoietin-2 (human) FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context.

Product References

Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells: K.L. Kim, et al.; Cardiovasc. Res. 72, 394 (2006)
Two natural eudesmane-type sesquiterpenes from Laggera alata inhibit angiogenesis and suppress breast cancer cell migration through VEGF- and Angiopoietin 2-mediated signaling pathways: N. Liang, et al.; Int. J. Oncol. 51, 213 (2017)

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Angiopoietin-2 (human) (rec.)