CD112R (mouse):Fc (human) (rec.)
Methods: Nectin-2/CD112 (mouse) (rec.) is coated on an ELISA plate at 1µg/ml. CD112R (mouse):Fc (human) (rec.) (AG-40B-0170) or an unrelated receptor fused to Fc (Control) is incubated (starting at 80nM with a twofold serial dilution). After incubation for 1h at RT, the binding is detected using an HRP-labeled anti-human Fc antibody.
|Synonyms||CD112 Receptor; Poliovirus Receptor-related Immunoglobulin Domain-containing Protein; Transmembrane Protein PVRIG|
|Source/Host||HEK 293 cells|
|Sequence||The extracellular domain of mouse CD112R (aa 28-165) is fused at the C-terminus to the Fc portion of human IgG1.|
|Biological Activity||Binds to the ligand mouse CD112 (PVRL2, Nectin-2) with an EC50 of 5nM.|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Concentration||0.1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 100μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
|Use/Stability||Stable for at least 6 months after receipt when stored at -20°C.|
|Product Specification Sheet|
CD112 (also called Nectin-2 or poliovirus receptor-related 2 protein) is a type I transmembrane glycoprotein and a member of the Ig gene superfamily (IGSF). CD112/Nectin-2 functions as an adhesion molecule involved in the formation of cell junctions and interactions with other Nectin-family molecules. Nectin-2 is a also a surface marker of some endothelial cells and regulates migration, proliferation and angiogenesis. CD112 functions as an immune checkpoint regulator by modulating T cell signaling, either as a costimulator of T cell function, or as a coinhibitor of T cells proliferation by binding to the receptors CD226 or TIGIT (T cell Ig and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain), respectively. The binding of CD112 ligand to these receptors is weak compared to CD155. Recently, a new receptor of CD112, called CD112R or PVRIG (Poliovirus receptor-related immunoglobulin domain-containing protein) has been characterized, showing high affinity to CD112. CD112R is a new coinhibitory receptor of the PVR family for human T cells, inhibiting TCR-mediated signals upon binding to CD112/Nectin-2. CD112R is preferentially expressed in T lymphocytes and NK cells. Cancer cells evade immune responses as a result of an immunosuppressive tumor microenvironment and CD112R, by playing a role in the regulation of the immune system, could be a new attractive cancer immunotherapy target.