AdipoGen Life Sciences

HpARI (Alarmin Release Inhibitor) (rec.) (His)

As low as CHF 360.00

In stock

AG-40B-0178-C05050 µgCHF 360.00

AG-40B-0178-30503 x 50 µgCHF 720.00

Figure 1: Binding of HpARI (Alarmin Release Inhibitor) (rec.) (His) (Prod. No. AG-40B-0178) to human and mouse IL-33.
Methods: IL-33 (human) (rec.) (untagged) (Prod. No. AG-40B-0038), IL-33 (mouse) (rec.) (untagged) (Prod. No. AG-40B-0041) as well as an unrelated control protein are coated on an ELISA plate at 1µg/ml. After blocking and washing steps, indicated concentrations of HpARI (Alarmin Release Inhibitor) (rec.) (His) are added. Following incubation for 1 h at RT, the binding was detected using an anti-His antibody (HRP).

Figure 2: Addition of HpARI (Alarmin Release Inhibitor) (rec.) (His) (Prod. No. AG-40B-0178) significantly reduces the effect of SW1116 colorectal cancer cells (CRC)-derived IL-33.
Method*: Groupe 2 Innate-lymphoid cells (ILC2s) are co-cultured with SW1116 CRC in RPMI medium. HpARI (Alarmin Release Inhibitor) (rec.) (His) (1µg/ml) is added or not in the medium for 24 hours. Frequencies of IL-13 positive ILC2s are measured by flow cytometry. Picture Courtesy of Giuseppe Ercolano, Camilla Jandus Lab, University of Geneva, Switzerland.
*For detailed method, see G, Ercolano, et al.; Nat. Comm. 12, 2538 (2021) [Lit. 3]

Specifications / Handling

More Information
Product Details
Synonyms	Heligmosomoides Polygyrus Alarmin Release Inhibitor
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	Heligmosomoides polygyrus protein HpARI (aa 17-251) is fused at the C-terminus to a His-tag.
Crossreactivity	Human Mouse
Specificity	Binds to human and mouse IL-33.
MW	~40kDa (SDS-PAGE); ~40 kDa (Monomer) (SEC)
Purity	≥90% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg purified protein (LAL test).
Concentration	After reconstitution: 1mg/ml
Reconstitution	Reconstitute with 50μl sterile water.
Formulation	Lyophilized. Contains PBS.
Other Product Data	Worm base HPBE_0000813301: Heligmosomoides polygyrus protein HpARI
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

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