HpARI (Alarmin Release Inhibitor) (rec.) (His)
Methods: IL-33 (human) (rec.) (untagged) (Prod. No. AG-40B-0038), IL-33 (mouse) (rec.) (untagged) (Prod. No. AG-40B-0041) as well as an unrelated control protein are coated on an ELISA plate at 1µg/ml. After blocking and washing steps, indicated concentrations of HpARI (Alarmin Release Inhibitor) (rec.) (His) are added. Following incubation for 1 h at RT, the binding was detected using an anti-His antibody (HRP).
Method*: Groupe 2 Innate-lymphoid cells (ILC2s) are co-cultured with SW1116 CRC in RPMI medium. HpARI (Alarmin Release Inhibitor) (rec.) (His) (1µg/ml) is added or not in the medium for 24 hours. Frequencies of IL-13 positive ILC2s are measured by flow cytometry. Picture Courtesy of Giuseppe Ercolano, Camilla Jandus Lab, University of Geneva, Switzerland.
*For detailed method, see G, Ercolano, et al.; Nat. Comm. 12, 2538 (2021) [Lit. 3]
|Synonyms||Heligmosomoides Polygyrus Alarmin Release Inhibitor|
|Source/Host||HEK 293 cells|
Heligmosomoides polygyrus protein HpARI (aa 17-251) is fused at the C-terminus to a His-tag.
Binds to human and mouse IL-33.
|MW||~40kDa (SDS-PAGE); ~40 kDa (Monomer) (SEC)|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test).|
|Concentration||After reconstitution: 1mg/ml|
|Reconstitution||Reconstitute with 50μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||
Worm base HPBE_0000813301: Heligmosomoides polygyrus protein HpARI
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. HpARI is a new type of reagent to prevent IL-33-mediated pathology.
- An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor: M. Suzukawa, et al.; J. Immunol. 181, 5981 (2008)
- HpARI protein secreted by a helminth parasite suppresses interleukin-33: M. Osbourne, et al.; Immunity 47, 739 (2017)
- PPARɣ drives IL-33-dependent ILC2 pro-tumoral functions: G. Ercolano, et al.; Nat. Commun. 12, 2538 (2021)