AdipoGen Life Sciences

HpARI (Alarmin Release Inhibitor) (rec.) (His)

CHF 360.00
In stock
AG-40B-0178-C05050 µgCHF 360.00
AG-40B-0178-30503 x 50 µgCHF 720.00
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Product Details
Synonyms Heligmosomoides Polygyrus Alarmin Release Inhibitor
Product Type Protein
Source/Host HEK 293 cells

Heligmosomoides polygyrus protein HpARI (aa 17-251) is fused at the C-terminus to a His-tag.

Crossreactivity Human

Binds to human and mouse IL-33.

MW ~40kDa (SDS-PAGE); ~40 kDa (Monomer) (SEC)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration After reconstitution: 1mg/ml
Reconstitution Reconstitute with 50μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data

Worm base HPBE_0000813301: Heligmosomoides polygyrus protein HpARI

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. HpARI is a new type of reagent to prevent IL-33-mediated pathology.

Product References
  1. An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor: M. Suzukawa, et al.; J. Immunol. 181, 5981 (2008)
  2. HpARI protein secreted by a helminth parasite suppresses interleukin-33: M. Osbourne, et al.; Immunity 47, 739 (2017)
  3. PPARɣ drives IL-33-dependent ILC2 pro-tumoral functions: G. Ercolano, et al.; Nat. Commun. 12, 2538 (2021)
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