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Figure 1: Immunoprecipitation of recombinant IL-33 proteins using anti-IL-33 (human), mAb (IL33026B) (Prod. No. AG-20A-0043). Recombinant IL-33 proteins at different concentrations were precipitated by anti-IL-33 mAb (IL33026B). The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot. 1. Control 100ng 2. IP : 1μg 3. IP : 500ng 4. IP : 250ng 5. IP : 125ng
Figure 2: Western blot analysis using anti-IL-33 (human), mAb (IL33026B) (Prod. No. AG-20A-0043) at 1:2'000 dilution. 1: Human IL-33 (His-tagged). 2: Human IL-33 (FLAG®-tagged). 3: Mouse IL-33 (FLAG®-tagged). 4: Other protein (His-tagged) (negative control).
ELISA: (direct or indirect: 1:2'000-1:5'000) Immunoprecipitation Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity
Human Mouse
Specificity
Recognizes human and mouse IL-33.
Purity Detail
Protein G-affinity purified.
Concentration
1mg/ml
Formulation
Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
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