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AdipoGen Life Sciences
VISTA (human):COMP (mouse) (rec.) (His)
480
CHF
CHF 480.00
In stock
AG-40B-0183-C05050 µgCHF 480.00
Figure 1: Recombinant VISTA (human):COMP (mouse) (rec.) (His) (Prod. No. AG-40B-0183) inhibits anti-CD3 antibody induced TNF-α secretion of human CD4+ T lymphocytes.
Method: Human CD4+ T cells are activated with coated anti-CD3 (human) (2.5µg/ml) and incubated for 5 days with VISTA (human):COMP (mouse) (rec.) (His) or Control protein (Prod. No. AG-35B-0007) (coated at 10µg/ml). TNF-α is measured from cell supernatants using the Cymax TNF-α (human) ELISA Kit (Prod. No. YIF-LF-EK0193).
Method: Human CD4+ T cells are activated with coated anti-CD3 (human) (2.5µg/ml) and incubated for 5 days with VISTA (human):COMP (mouse) (rec.) (His) or Control protein (Prod. No. AG-35B-0007) (coated at 10µg/ml). TNF-α is measured from cell supernatants using the Cymax TNF-α (human) ELISA Kit (Prod. No. YIF-LF-EK0193).
| Product Details | |
|---|---|
| Synonyms | V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation; Platelet Receptor Gi24; Stress-induced Secreted Protein-1; Sisp-1; V-set Domain-containing Immunoregulatory Receptor |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
The extracellular domain of human VISTA (aa 33-194) is fused at the C-terminus to the domain of the mouse cartilage oligomeric matrix protein (COMP) (aa 28-72) and to a His-tag. |
| Crossreactivity | Human |
| MW | ~40-55kDa (SDS-PAGE) and >500kDa (Size Exclusion Chromatography (SEC)) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50μl sterile water. |
| Accession Number | Q9H7M9 |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
UniProt link Q9H7M9: VISTA (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Human VISTA is a 55 to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells. Recently, it has been reported that the recombinant VISTA protein needs to be multimerized to be active as soluble ligand. The protein VISTA (human):COMP (mouse) (with the extracellular domain of VISTA (human) fused to the pentamerization domain from the cartilage oligomeric matrix protein (COMP)), but not VISTA-Fc, functions as an immunosuppressive agonist in vivo inhibiting the proliferation of CD4+ T cells.
Product References
- VISTA.COMP - an engineered checkpoint receptor agonist that potently suppresses T cell-mediated immune responses: A. Prodeus, et al.; JCI Insight 2, e94308 (2017)
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