VISTA (mouse):COMP (mouse) (rec.) (His)

CHF 520.00
In stock
AG-40B-0181-C05050 µgCHF 520.00
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Product Details
Synonyms V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation; Platelet Receptor Gi24; Stress-induced Secreted Protein-1; Sisp-1; V-set Domain-containing Immunoregulatory Receptor
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

The extracellular domain of mouse VISTA (aa 33-191) is fused at the C-terminus to the domain of the mouse cartilage oligomeric matrix protein (COMP) (aa 28-72) and a His Tag.

Crossreactivity Mouse
MW ~35-40kDa (SDS-PAGE) and >250kDa (Size Exclusion Chromatography (SEC))
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 50μl sterile & endotoxin-free water.
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link Q9D659: VISTA (mouse)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Mouse VISTA is a 55 to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells. Recently, it has been reported that recombinant VISTA protein needs to be multimerized to be active as soluble ligand. The protein VISTA (mouse):COMP (mouse) (with the extracellular domain of VISTA (mouse) fused to the pentamerization domain from the cartilage oligomeric matrix protein (COMP)), but not VISTA-Fc, functions as an immunosuppressive agonist in vivo inhibiting the proliferation of CD4+ T cells. 

Product References
  1. VISTA.COMP - an engineered checkpoint receptor agonist that potently suppresses T cell-mediated immune responses: A. Prodeus, et al.; JCI Insight 2, e94308 (2017)
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