IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.)
|Synonyms||IL-2 Switch-2 (monomeric):Fc Knobs-into-Holes (human) (rec.); Interleukin-2; T Cell Growth Factor; TCGF; IL-2 pH resistant; pH-selective IL-2 Mutein|
|Source/Host||HEK 293 cells|
The extracellular domain of human IL-2 (aa 21-153) (mutant pH-resistant Switch-2 containing mutations T37H, R38L, T41S, F42Y, and K43G) is fused at the C-terminus to the Fc portion of human IgG1 (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).
|MW||~45kDa and 28kDa (SDS-PAGE)|
|Endotoxin Content||<0.01EU/μg protein (LAL test).|
for 10µg size: 0.1mg/ml
for 100µg size: 1mg/ml
|Reconstitution||Reconstitute with 100μl endotoxin-free water.|
|Formulation||Lyophilized. Contains PBS.|
|Protein Negative Control|
|Other Product Data||
Uniprot link P60568 : IL-2 (human)
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.
IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Rα (CD25), IL-2Rβ and IL-2Rγ. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rβ and IL-2Rγ. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rβ and IL-2Rγ receptors.
Activity of interleukin-2 (IL-2), is profoundly affected by pH, limiting IL-2 signaling within the acidic environment of tumors. A new designed IL-2, named Switch-2, binds the IL-2 receptor subunit IL-2Rα with higher affinity, triggers STAT5 activation and drives CD8+ T cell effector function more potently at acidic pH than at neutral pH.
The protein IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-2 (human) (Switch-2):Fc Knobs sequence (synthesizing a protein of 45kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.).