Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.)
440
CHF
CHF 440.00
In stock
AG-40B-0234-C01010 µgCHF 440.00
AG-40B-0234-30103 x 10 µgCHF 880.00
AG-40B-0234-C100100 µgCHF 1'800.00
Figure 1: Protein structure of IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0234).
| Product Details | |
|---|---|
| Synonyms | IL-2 Switch-2 (monomeric):Fc Knobs-into-Holes (human) (rec.); Interleukin-2; T Cell Growth Factor; TCGF; IL-2 pH resistant; pH-selective IL-2 Mutein |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
The extracellular domain of human IL-2 (aa 21-153) (mutant pH-resistant Switch-2 containing mutations T37H, R38L, T41S, F42Y, and K43G) is fused at the C-terminus to the Fc portion of human IgG1 (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity |
Human Mouse |
| MW | ~45kDa and 28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg protein (LAL test). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml |
| Reconstitution | Reconstitute with 100μl endotoxin-free water. |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
Uniprot link P60568 : IL-2 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.
IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Rα (CD25), IL-2Rβ and IL-2Rγ. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rβ and IL-2Rγ. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rβ and IL-2Rγ receptors.
Activity of interleukin-2 (IL-2), is profoundly affected by pH, limiting IL-2 signaling within the acidic environment of tumors. A new designed IL-2, named Switch-2, binds the IL-2 receptor subunit IL-2Rα with higher affinity, triggers STAT5 activation and drives CD8+ T cell effector function more potently at acidic pH than at neutral pH.
The protein IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-2 (human) (Switch-2):Fc Knobs sequence (synthesizing a protein of 45kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.).
Related Products
