AdipoGen Life Sciences

Flagellin (rec.) (untagged) (highly active)

CHF 380.00
In stock
AG-40B-0243-C05050 µgCHF 380.00
More Information
Product Details
Synonyms FliC
Product Type Protein
Source/Host E. coli

Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495).

Crossreactivity Human

Binds to human and mouse TLR5 (Toll-like receptor 5).

Biological Activity

Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, β-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1µg/mouse.

MW ~54kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.001EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 50μl sterile water.
Accession Number P06179
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link P06179: Flagellin (Salmonella typhimurium)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.