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AdipoGen Life Sciences
IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.)
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AG-40B-0252-C05050 µgINQ
Figure 1: Protein structure of IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0252) containing a single heterodimer.
| Product Details | |
|---|---|
| Synonyms | InVivoKine™; Interleukin-35 (human):Fc Knobs-into-Holes (human) (rec.); IL-12 Subunit α; EBI3; Interleukin-27 Subunit β; Ebi3; Epstein-Barr Virus-induced Gene 3 Protein Homolog |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
IL-12Ap35 (aa 23-219) (human):Fc (LALA-PG) Knobs and IL-27B/EBI3 (aa 21-229) (human):Fc (LALA-PG) Holes form the IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity | Human |
| Specificity |
Binds to human IL-35 receptor complex. Fc (LALA-PG)-KIH (human) does not bind to FcRs. |
| MW | ~68kDa and 60 kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.005 EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50μl endotoxin-free water. |
| Accession Number | P29459 / Q14213 |
| Formulation | Lyophilized. Contains PBS |
| Protein Negative Control |
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018) |
| Other Product Data |
UniProt link P29459: IL-12A (human) UniProt link Q14213: IL-27B /Ebi3 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-12 (IL-12) family members are heterodimer glycoproteins, composed of two covalently linked subunits, α and β chains. The α-subunit consists of IL-23p19, IL-27p28, and IL-12p35 and the β-subunit includes IL-12p40 and Epstein-Barr virus-induced gene (Ebi3). IL-12 members bind to cognate heterodimeric receptor chains expressed on T cells.
This family includes IL-12, IL-23, IL-27 and IL-35 and IL-39. IL-12 and IL-23 are predominantly proinflammatory cytokines that contribute key roles in the development of Th1 and Th17 cells, respectively. IL-27 has both pro- and anti-inflammatory properties and is a potent T cell immunomodulator. IL-35, a new member of this family, is a potent inhibitory cytokine produced by natural, thymus-derived regulatory T cell (nTreg) populations. Recently, a subset of B cells that produce IL-35 was identified and shown to provide protection against autoimmune diseases. These IL-12 family members link innate immunity with the development of adaptive immunity and are also important for regulating T cell responses.
IL-35 is composed of two subunits, IL-12A and Epstein-Barr virus-induced gene 3 (EBI3) signals via a heterodimeric receptor consisting of IL-12Rβ2 and glycoprotein (gp130), leading to activation of STAT 1 and 4. IL-35 is a potent suppressive cytokine directly limiting the inflammatory response that has important roles in infection, cancer and autoimmune diseases.
The protein IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-12A:Fc Knobs sequence (synthesizing a protein of 68kDa) and one encoding for the IL27B/EBI3:Fc Holes sequence (synthesizing a protein of 60kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-35 (human):Fc (LALA-PG)-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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