AdipoGen Life Sciences

IL-11 (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.)

CHF 440.00
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AG-40B-0266-C05050 µgCHF 440.00
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Product Details
Synonyms InVivoKine™; IL-11 (human) (monomeric):Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Interleukin-11; Adipogenesis Inhibitory Factor; AGIF
Product Type Protein
Properties
Source/Host HEK 293 cells. Produced using animal component-free medium.
Sequence

Human IL-11 (aa 22-199) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).

Crossreactivity Human
Mouse
MW ~ 50 kDa and 28kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.002 EU/μg protein (LAL test).
Concentration After reconstitution: 1mg/ml
Reconstitution Reconstitute with 50μl endotoxin-free water.
Accession Number P20809
Formulation Lyophilized. Contains PBS.
Protein Negative Control

Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018)

Other Product Data

Uniprot link P20809: IL-11 (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Interleukin-11 (IL-11) is a 19 kDa monomeric protein. IL-11 forms a hexameric signaling complex, which consists of the cytokine, the cognate receptor (IL-11Rα) and the common signaling receptor (GP130) in a 2:2:2 stoichiometry. Interleukin-11 (IL-11) belongs to the IL-6 family of cytokines, which includes IL-6, leukemia inhibitory factor, Oncostatin M, ciliary neurotrophic factor, cardiotrophin-1, cardiotrophin-like cytokine, neuropoietin, IL-27 and IL-31.

Interleukin-11 (IL-11) has diverse biological activities: i) it stimulates the proliferation and differentiation of hematopoietic stem cells and progenitor cells,  enhances the production of megakaryocytes, leading to increased platelet production (thrombopoiesis);  ii)  IL-11 activates fibroblasts and exhibits either a pro-inflammatory or pro-fibrotic phenotype. Recently, IL-11 has been shown to be a key factor in 'inflammaging' (chronic inflammation), one of the hallmarks of aging leading to age-related illnesses, including cardiometabolic, neurodegenerative, musculoskeletal dysfunction and cancer. Inhibition of IL-11 has been shown to increase lifespan and health span in mice. IL-11 influences aging by inducing IL-33 in fibroblasts and NLRP3 in macrophages.

The protein IL-11 (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-11 (human):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 50 kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-11 (human) (monomeric):Fc-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

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