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AdipoGen Life Sciences
IL-4 (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.)
440
CHF
CHF 440.00
In stock
AG-40B-0261-C05050 µgCHF 440.00
NEW
Figure 1: Protein structure of IL-4 (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0261).
| Product Details | |
|---|---|
| Synonyms | InVivoKine™; IL-4 (human) (monomeric):Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Interleukin-4; B Cell Stimulatory Factor 1; BSF-1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
Human IL-4 (aa 25-153) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity | Human |
| MW | ~50kDa and 28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.002 EU/μg protein (LAL test). |
| Concentration | After reconstitution: 1mg/ml |
| Reconstitution | Reconstitute with 50μl endotoxin-free water. |
| Accession Number | P05112 |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control |
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018) |
| Other Product Data |
Uniprot link P05112: IL-4 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-4 (IL-4) is a monomeric TH2 cytokine of approximately 17 kDa that shows pleiotropic effects during immune responses. It functions as a potent regulator of immunity. IL-4 was first identified as a T cell-derived growth factor for B cells. IL-4 and IL-13 activate many common signaling pathways. IL-4 is mainly produced by Th2 cells, but can also be secreted by natural killer T (NKT) cells, mast cells, eosinophils, basophils, and innate-type lymphoid cells. IL-4 can signal through the type II IL-4 receptor, which is a heterodimer of IL-4Rα and IL-13Rα1 chains, and also through the type I IL-4 receptor, which is a heterodimer of IL-4 receptor alpha (IL-4Rα) and common γ(γc) chains. IL-4 binding results in tyrosine kinase Janus kinase 1 (JAK1) and JAK3 activation.
IL-4 and IL-13 may be involved at several levels of the fibrotic process since they activate the fibroblasts that differentiate into myofibroblasts, and they stimulate extracellular matrix (ECM) production and deposition. IL-4 functions as a co-mitogen of B-cells. It is important in leukocyte survival, Th2 cell-mediated immunity, IgE class switching in B cells, and tissue repair and homeostasis through “alternative” macrophage activation.
The protein IL-4 (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-4 (human):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 50kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-4 (human) (monomeric):Fc-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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